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Kit for screening liver cancer by urine

A technology of kits and reagents, which is applied in the field of medical testing, can solve the problems of little diagnostic significance, lack of kits, and high detection rate of missed diagnosis, and achieve the effects of saving detection costs, convenient collection, and high sensitivity

Pending Publication Date: 2020-04-17
大连德泰克森生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing kits for detection of liver cancer markers are of little significance in the early diagnosis of cancer. After positive results, the cancer has often developed to the middle and late stages, and the degree of cancer invasion cannot be effectively determined. Moreover, the detection rate of missed diagnosis is relatively low. Big
Therefore, there is still a lack of very effective kits in this field, especially the lack of detection methods that are of great significance for the early screening of liver cancer

Method used

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  • Kit for screening liver cancer by urine
  • Kit for screening liver cancer by urine
  • Kit for screening liver cancer by urine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] 1. Operation steps

[0052] 1. Adding samples: set blank wells, standard wells and sample wells to be tested respectively, and add samples according to Table 1 for the following operations.

[0053] 2. Add 20 μL of distilled water to the blank well of the ELISA plate, add 20 μL of IAA standard solution to the standard well first, and then add 20 μl of the urine sample to be tested to the sample well to be tested. Add 50 μL of phosphate buffer solution to the standard well and the sample well to be tested respectively. When adding the sample, add the sample to the bottom of the well of the microtiter plate, try not to touch the wall of the well, shake gently to mix.

[0054] 3. Incubation: Seal the plate with a sealing film and incubate at 37°C for 10 minutes.

[0055] 4. Preparation of R reagent mixture: mix reagent R 1 and R 2 Prepare it for later use, according to the volume ratio R1:R2=1:50, mix it before use, and store it away from light.

[0056] 5. Operation:...

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Abstract

The invention discloses a kit for screening liver cancer by urine. The kit comprises a phosphate buffer solution, a reagent R1, a reagent R2, a reagent R3, a reagent R4, 5-oxindole acetate oxidase anda 5-hydroxyindoleacetic acid (IAA) standard substance, wherein the reagent R1 is a FeCI3 or Fe2(SO4)3 solution, the reagent R2 is a perchloric acid or H2SO4 solution, the reagent R3 is a MnCI2 or MnSO4 solution, and the reagent R4 is a tetrachlorophenol solution. The kit provided by the invention can be used for quantitative marker detection of human liver cancer urine, and has high sensitivity.The kit disclosed by the invention uses trace urine as a detection specimen, and has the advantages of convenience in collection, detection cost saving and the like.

Description

technical field [0001] The patent of the invention relates to the field of medical testing, in particular to a kit for urine detection and screening of liver cancer. Background technique [0002] Liver cancer is one of the most common malignant tumors in China, and its incidence is still on the rise in recent years. Early diagnosis of patients with better prognosis, postoperative 5-year survival rate increased. Liver cancer refers to malignant tumors that occur in the liver, including primary liver cancer and metastatic liver cancer. People usually refer to primary liver cancer when they say liver cancer. Primary liver cancer is one of the most common malignant tumors in clinical practice. According to the latest statistics, there are about 800,000 new liver cancer patients in the world every year, ranking fifth among malignant tumors. According to cell type, primary liver cancer can be divided into hepatocellular carcinoma, cholangiocellular carcinoma and mixed liver carc...

Claims

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Application Information

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IPC IPC(8): C12Q1/26
CPCC12Q1/26
Inventor 王亚东张晓辉王若雨周士胜
Owner 大连德泰克森生物医药有限公司
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