Cell culture plate, manufacturing method thereof, and cell patch separating method

A technology of cell culture and manufacturing method, which is applied in the field of cell culture plates, can solve the problems of damaged cell activity and quality, and achieve the effect of ensuring activity and quality

Inactive Publication Date: 2019-03-05
HONG FU JIN PRECISION IND (SHENZHEN) CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the process of slowly lowering the temperature can damage the viability and quality of the cells

Method used

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  • Cell culture plate, manufacturing method thereof, and cell patch separating method
  • Cell culture plate, manufacturing method thereof, and cell patch separating method

Examples

Experimental program
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Embodiment Construction

[0023] The invention provides a cell culture plate capable of ensuring the activity and quality of cells when the cells are separated from the cell culture plate.

[0024] A cell culture plate, the cell culture plate includes a base plate and a photodisruption layer formed on the surface of the base plate.

[0025] The material of the substrate is selected from one of transparent plastic and glass, such as polystyrene.

[0026] The photo-breakable layer includes several photo-breakable groups, and the photo-breakable groups are bonded to the surface of the substrate through amido groups. The chemical structural formula of the photocleavage group is Among them, R 1 group and R 3 The group is an alkane group, R 2 The group includes at least an alkane group or an alkene group, R 5 group, R 6 group and R 7 The groups are respectively one of a hydrogen group and an alkane group. Under the light, the ester group on the ortho position of the nitro group in the photocleavage ...

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PUM

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Abstract

A cell culture plate comprising a substrate and a photoinduced fracture layer formed on one surface of the substrate is disclosed. The photoinduced fracture layer includes a plurality of photoinducedfracture groups having a chemical structure shown in the description, wherein R1 and R3 are alkyl, R2 at least includes alkyl or alkylene, R5, R6 and R7 are one of hydrogen and alkyl, and the photoinduced fracture groups are bonded to the surface through acylamino.

Description

technical field [0001] The present invention relates to a cell culture plate, and also relates to a method for manufacturing the above cell culture plate and a method for separating cell membranes using the above cell culture plate. Background technique [0002] In the prior art, a temperature-sensitive cell culture plate is usually used, and the cell culture plate is usually formed by grafting poly-N-isopropylacrylamide on a polystyrene substrate, and during the cell culture process , at 37 degrees Celsius for cell culture, when the cell culture is over, it is necessary to slowly reduce the temperature to 20 degrees Celsius for cell separation. However, the process of slowly lowering the temperature can damage the viability and quality of the cells. Therefore, it is necessary to provide a cell culture plate capable of ensuring the activity and quality of the cells when the cells are separated from the cell culture plate. Contents of the invention [0003] The present in...

Claims

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Application Information

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IPC IPC(8): C12M3/04C08J7/12C08L33/24C12N5/00
CPCC12M25/06C08J7/12C08J2333/24C12N5/00C12N2509/10C12N2533/30C12N2539/10
Inventor 简秀纹
Owner HONG FU JIN PRECISION IND (SHENZHEN) CO LTD
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