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A culture method for prolonging the storage period of kiwi fruit tissue culture seedlings

A cultivation method and a technology of tissue culture seedlings, which are applied in the cultivation field of prolonging the storage period of kiwifruit tissue culture seedlings, can solve the problems of costing a lot of manpower, material resources and time, increasing the probability of variation of culture materials, unfavorable preservation of original germplasm, etc., and achieve application The effect of high value, saving tissue culture cost, and reducing nutrient consumption

Active Publication Date: 2022-06-21
POMOLOGY RES INST FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Kiwifruit tissue culture seedlings are generally subcultured once a month, and can be stored for up to 2 months. Frequent subculture requires a lot of manpower, material resources and time; at the same time, as the number of subcultures increases, the probability of variation of culture materials also increases. increase, which is not conducive to the preservation of the original germplasm

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  • A culture method for prolonging the storage period of kiwi fruit tissue culture seedlings

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Embodiment 1

[0020] A culture method for prolonging the preservation period of kiwifruit tissue culture seedlings, comprising the following steps:

[0021] (1) According to MS+6-BA 1.0 mg / L+NAA 0.5 mg / L+GA 3 0.1 mg / L + agar 7 g / L + sucrose 15 g / L to prepare a special subculture medium; adjust the pH to 6.0 before the medium is sterilized;

[0022] (2) Select non-polluting, robust kiwifruit tissue culture seedlings to inoculate in the above-mentioned medium;

[0023] (3) The inoculated kiwifruit tissue culture seedlings should be stored in a culture room with a culture temperature of 25±1°C, a light time of 12 h / d, and a light intensity of 1500-2000 lx;

[0024] (4) The tissue culture seedlings with delayed growth can be maintained for 5 months. During the period, when the leaves of the tissue culture seedlings are found to be dry, the new medium should be replaced in time. The survival rate of the cultured tissue culture seedlings was 86%.

Embodiment 2

[0026] A culture method for prolonging the preservation period of kiwifruit tissue culture seedlings, comprising the following steps:

[0027] (1) According to MS+6-BA 1.0 mg / L +NAA 1.0 mg / L+GA 3 0.1 mg / L + agar 7 g / L + sucrose 20 g / L to prepare a special subculture medium; adjust the pH to 6.0 before the medium is sterilized;

[0028] (2) Select non-polluting, robust kiwifruit tissue culture seedlings to inoculate in the above-mentioned medium;

[0029] (3) The inoculated kiwifruit tissue culture seedlings should be stored in a culture room with a culture temperature of 25±1°C, a light time of 12 h / d, and a light intensity of 1500-2000 lx;

[0030] (4) The tissue culture seedlings with delayed growth can be maintained for 4.5 months. During the period, when the leaves of the tissue culture seedlings are found to be dry, the new medium should be replaced in time. The survival rate of the cultured tissue culture seedlings was 83%.

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Abstract

The invention discloses a cultivation method for prolonging the preservation period of kiwifruit tissue culture seedlings, which is to add a certain amount of sucrose on the basis of the traditional subculture medium of kiwifruit tissue culture seedlings, so as to effectively prolong the preservation time of tissue culture seedlings and solve the current problem The short storage period of kiwifruit tissue culture seedlings. Carrying out the preservation of the kiwifruit tissue culture seedlings according to the method of the present invention can generally delay the growth of the tissue culture seedlings for 4-5 months, and the survival rate of the tissue culture seedlings can reach more than 80%.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a culture method for prolonging the preservation period of kiwifruit tissue culture seedlings. Background technique [0002] Kiwifruit is rich in nutrients and contains a variety of vitamins, fats and proteins. Among them, the content of vitamin C is 100mg-400mg / 100g, which is much higher than that of citrus, pear and apple. It is known as the "King of Vc". In addition to the edible value of kiwi, its flowers are beautiful and fragrant, and have high ornamental value. my country is the center of the distribution of kiwifruit resources. The germplasm resources are abundant and widely distributed, but they have been in wild or semi-wild state for a long time. Due to the deterioration of the ecological environment, some species are already in an endangered situation, so collection and preservation are urgently needed. [0003] Commonly used preservation methods include...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 高敏霞陈义挺冯新赖瑞联陈文光
Owner POMOLOGY RES INST FUJIAN ACAD OF AGRI SCI