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Fungal enzyme for improving content of gasifiable oil in crude oil as well as preparation method and application method thereof

A fungal enzyme, gasification oil technology, applied in the field of microorganisms, can solve problems such as unclear conversion rate

Pending Publication Date: 2019-03-19
陕西博秦生物工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the conversion rate of fungal enzymes to degrade large molecular components in crude oil into small molecular gasifiable components is not yet clear, and whether heavy components such as asphaltene can be converted into saturated hydrocarbons remains to be confirmed

Method used

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  • Fungal enzyme for improving content of gasifiable oil in crude oil as well as preparation method and application method thereof
  • Fungal enzyme for improving content of gasifiable oil in crude oil as well as preparation method and application method thereof
  • Fungal enzyme for improving content of gasifiable oil in crude oil as well as preparation method and application method thereof

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Embodiment Construction

[0034] Embodiments of the present invention will be described in detail below in conjunction with examples, but those skilled in the art will understand that the following examples are only used to illustrate the present invention, and should not be considered as limiting the scope of the present invention.

[0035] 1 Materials and methods

[0036] 1.1 Materials

[0037] (1) Tested strains: a total of 4 strains, Z3 is Aspergillus oryzae, the accession number on Genbank is KT189153, and it was preserved in the China Center for Type Culture Collection on December 30, 2016, and the preservation number is CCTCC NO: M2016789, The deposit address is Wuhan University, China; Z05 is Aspergillus spelunceus, the accession number on Genbank is KT189154; Z06 is Aphanocladium aranearum, the accession number on Genbank is KT189155; Z10 is Aspergillus sydowii, the accession number on Genbank is KT189156. The tested strains were all isolated and screened by the Microbial Resources Laboratory...

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Abstract

The invention discloses a fungal enzyme for improving the content of gasifiable oil in crude oil as well as a preparation method and an application method thereof. The fungal enzyme is prepared from the following raw materials: an oil displacement fungus and an enzyme-production culture medium, wherein the enzyme-production culture medium is prepared from wheat bran, a salt solution and the crudeoil. The fungal enzyme disclosed by the invention is used for carrying out enzymatic conversion on the crude oil and a macromolecular component including asphalt in the crude oil is degraded and converted into a small molecular gasifiable component; the content of saturated hydrocarbon and aromatic hydrocarbon in an enzymatic conversion product of the crude oil is remarkably improved; the preparation method is simple and easy to operate and implement; and the fungal enzyme can provide theoretical support for improving the quality of the crude oil by utilizing the fungal enzyme and also provides scientific evidence for a technological deign for improving the yield of gasoline and low-boiling-point coal diesel in a refining process of the crude oil by utilizing a fungal enzyme method.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a fungal enzyme for increasing the content of gasifiable oil in crude oil, a preparation method and a use method thereof. Background technique [0002] Gasoline, kerosene and diesel oil are all gasifiable oils in crude oil, the main components are saturated hydrocarbons and some aromatic hydrocarbons, and their boiling ranges are 34°C-205°C, 180°C-310°C and 180°C-370°C respectively. The gasifiable oil in the present invention refers to gasoline and part of low-boiling kerosene and diesel oil that can be gasified at 230° C. in crude oil. Gasoline, kerosene and diesel are the blood of industry and transportation, and are closely related to human life. Under the condition of increasingly depleted oil resources, improving the utilization rate of crude oil is one of the important ways to solve the shortage of oil resources. If the high-molecular components in crude oil can be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/00C12N9/04C12P5/00C12R1/69C12R1/66
CPCC12N9/00C12N9/0006C12P5/00C12P5/005
Inventor 高卉薛泉宏来航线
Owner 陕西博秦生物工程有限公司
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