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Urine protein marker for metastatic encephaloma and application of marker

A technology for brain metastases and uses, applied in the field of protein markers, can solve the problems of lack of certainty criteria for differential diagnosis, different treatment and prognosis methods, etc.

Inactive Publication Date: 2019-03-19
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Clinically, some solitary brain metastases and gliomas with unclear primary disease history have similar clinical manifestations and MRI routine examination findings, but the treatment and prognosis of the two are quite different. Extremely important but lacks definitive criteria

Method used

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  • Urine protein marker for metastatic encephaloma and application of marker
  • Urine protein marker for metastatic encephaloma and application of marker
  • Urine protein marker for metastatic encephaloma and application of marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Establishment of a local growth rat model of brain metastases

[0044] Walker-256 rat breast cancer cell line has good tumorigenicity and is applied in the research of brain metastasis (Hasegawa H et al. Changes of the blood-brain barrier in experimental metastatic brain tumors.J Neurosurg,1983,59(2 ):304-10). Direct intracerebral injection of tumor cells can detect the growth of tumor cells in the brain, which is the easiest method to control tumor growth and the highest reproducibility in animal models of brain metastases (Lewis K M et al Characterization of Walker 256 breast carcinoma cells from twotumour cell banks as assessed using two models of secondary brain tumors [J]. Cancer Cell Int, 2013, 13(1): 5). By injecting the Walker-256 cell line into the rat brain to simulate the local tumor growth process of brain metastases, it has important guiding significance for clinical diagnosis and monitoring of disease progression.

[0045] In the present inven...

Embodiment 2

[0063] Example 2: Identification of protein markers

[0064] 1. Urinary protein extraction and storage: centrifuge the urine at 2000g at 4°C for 30 minutes, take the supernatant, put it in a new EP tube, and continue to centrifuge at 12000g at 4°C for 30 minutes; take the supernatant and store it at -80°C.

[0065] Urine protein was precipitated with ethanol, and the protein concentration was measured by Bradford method, followed by enzyme digestion on the membrane. See WisniewskiJR, Zougman A, Nagaraj N, Mann M. Universal sample preparation method for proteome analysis. Nature methods 2009; 6:359-62. The BCA method was used to measure the concentration of peptides.

[0066] 2. LC-MS / MS tandem mass spectrometry analysis:

[0067]Dilute the peptide sample to 0.5 μg / μL with 0.1% formic acid. Peptide samples were separated by Thermo liquid phase system EASY-nLC 1200 loading system. The elution time is 120 minutes, and the column flow rate is 0.3 μl / min. The elution gradient i...

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Abstract

The invention relates to a urine protein marker for metastatic encephaloma and an application of the marker, in particular to an application of the urine protein marker acquired by a local growth model of the metastatic encephaloma and mass spectrometry to early diagnosis of the metastatic encephaloma and differential diagnosis of brain glioma. The urine protein marker is selected from an NKG2-D II-type integrated film protein, a galactoagglutinin-3 binding protein, a complement C4, a macrophage colony stimulating factor 1, superoxide dismutase [Cu-Zn], a tumor necrosis factor receptor super-family of steroid member 1 B, a pancreatic stone protein, an intercellular adhesion molecule 1, a vascular cell adhesion protein and the like. According to the urine protein marker, an acquired differential protein in urine provides a noninvasive, simple and convenient selection for early diagnosis of the metastatic encephaloma, differential diagnosis of brain glioma and follow-up treatment.

Description

technical field [0001] The invention relates to clinical medicine, in particular to protein markers related to human brain metastases. Specifically, the present invention relates to the early diagnosis of human brain metastases obtained by using the local growth model of Walker-256 tumor cells in the rat brain and mass spectrometry analysis, and the use of protein markers related to the differential diagnosis of brain gliomas. Background technique [0002] Biomarkers refer to monitorable changes related to physiological or pathological processes, which cover a wide range and are currently the most widely used and most influential markers for early diagnosis or prognosis of diseases. Disease markers play an important role in the prevention, diagnosis, treatment and prognosis of diseases, and can indicate the occurrence and progress of diseases early and sensitively. Urine is the excrement excreted from the body through the urinary system and urinary tract. It is rich in vari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N27/62G01N33/574
CPCG01N33/6848G01N27/624G01N33/574
Inventor 高友鹤张琳珮李育秋孟文书
Owner BEIJING NORMAL UNIVERSITY
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