Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for stabilizing proteins

A stabilized protein technology, applied in the field of stabilized proteins, can solve problems such as damage to protein function and stability, unrevealed polypeptide dissociation events, etc.

Pending Publication Date: 2019-03-19
隆扎有限公司
View PDF22 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Peptide dissociation events not revealed by other quality control tests
[0005] In addition, product dissociation events can impair the function and stability of expressed proteins

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for stabilizing proteins
  • Method for stabilizing proteins
  • Method for stabilizing proteins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0474] Example 1. Cooling Cells Alleviates Hypoxia Associated with Harvesting and Storage of Cell Samples

[0475] Oxygen depletion is the underlying cause of dissociations that have been identified and documented. The depletion rate decreases with temperature. figure 1 Show O 2 Solubility increases inversely with temperature (compare 37°C, 15°C and 5°C). Changes in three other oxygen-related parameters at these three temperatures are also shown. It was therefore hypothesized that cooling the bioreactor / sample could similarly help prevent dissociation.

[0476] Mitigation strategies including cell cooling for bioreactors, primary storage and storage bags or bottles such as figure 2 shown.

Embodiment 2

[0477] Example 2. Harvest profile of two bioreactors with and without harvest aeration

[0478] Harvest profiles for the two bioreactors with and without harvest aeration are shown in image 3 middle. The data show hypoxic events (near 0%) in the culture during harvest, consistent with the dissociation hypothesis. In contrast, profiles of cultures used to maintain oxygenation (~20% at nadir) during harvest by performing the mitigation strategy as shown are shown.

Embodiment 3

[0479] Example 3. Non-reducing SDS polyacrylamide gels with and without moderation

[0480] Figure 4 is a schematic representation of non-reducing SDS polyacrylamide gel samples prepared without tempering (left panel) and with tempering (right panel) for IgGl monoclonal antibodies. For samples prepared without tempering, multiple bands were observed, indicating dissociated polypeptide species. On the contrary, for the figure 2 For samples with the mitigation strategy shown in , a single high molecular weight band with no evidence of dissociation was detected. These results suggest that palliative strategies including cooling cells inhibit protein dissociation.

[0481]No simple trends were observed between individual growth characteristics (maximum viable cell concentration, harvested viable cell concentration, IVC, viability at harvest) and dissociation. The absence of a trend does not rule out the possibility of an interaction between a growth parameter and another p...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present disclosure features methods and compositions for increasing the amount of products of cellular metabolism, e.g., proteins, by lowering the temperature of cells expressing the product at one or more steps while culturing the cells, expressing the product, and / or recovering the product.

Description

[0001] related application [0002] This application claims priority to US Serial No.: 62 / 348595 filed June 10, 2016, the entire contents of which are incorporated herein by reference in their entirety. field of invention [0003] The present disclosure relates to methods and compositions for inhibiting the dissociation of cellular products (eg, polypeptides) expressed in cultured cells. Background of the invention [0004] Cellular products such as recombinant therapeutic proteins are typically expressed in cellular expression systems, such as mammalian cell expression systems. Hundreds of market-approved biologic drugs are expressed in mammalian cell lines. However, the high costs associated with its production contribute to increasing global health costs. Product dissociation events can result in less than expected stability or activity of proteins or polypeptides expressed from cells grown in cell culture. These events were detected by non-reducing SDS electrophoretic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P21/00
CPCC12P21/02A61K38/00A61P43/00C12P21/00A61K2039/505C07K16/00C12M27/02C12M41/34C07K1/14C07K2317/56C12M41/28C07K16/065C12M29/00C12M47/10C07K2317/14C12M41/12C12M47/20
Inventor M.可汗A.波特
Owner 隆扎有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com