A method for fermenting and extracting and purifying L-leucine

A technology of leucine and fermented liquid, applied in the direction of chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of high product cost, low efficiency, large demand for personnel, etc., and improve production efficiency, increase fermentation yield, and optimize the effect of fermentation culture steps

Active Publication Date: 2021-06-29
XINJIANG FUFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the drying technology of L-leucine at home and abroad basically adopts the double-cone drying method. Although this method can meet the drying requirements of L-leucine, the product cost is high, the efficiency is low, and the demand for personnel is large.

Method used

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  • A method for fermenting and extracting and purifying L-leucine
  • A method for fermenting and extracting and purifying L-leucine
  • A method for fermenting and extracting and purifying L-leucine

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A method for fermenting and extracting and purifying L-leucine, comprising the steps of:

[0029] Components of the fermentation medium: glucose 50g / L, (NH 4 ) 2 SO 4 10g / L, corn syrup 10g / L, KH 2 PO 4 4g / L, MgSO 4 ·7H 2 O 1.5g / L, citric acid 0.5g / L, MnSO 4 ·H 2 O 10mg / L, FeSO 4 ·7H 2 O 0.1g / L, V B1 5mg / L, V H 0.1mg / L.

[0030] The components of the compound amino acid solution: glutamic acid 10g / L, isoleucine 5 / L, valine 5g / L,

[0031] Components of nutrient solution: glucose 100g / L, glycerin 5g / L, betaine 1g / L;

[0032] Step 1) Taking Corynebacterium glutamicum CGMCC No.7033 as an example, the seed solution (OD 600 value is 9.5) into the fermentation medium, the culture temperature is 36.8°C, the pH of the medium is controlled by adding 20% ​​ammonia water automatically to be 7.2, and the dissolved oxygen is controlled at 25% by stirring and ventilation; when the fermentation reaches 12h, the compound Amino acid solution is fed into the tank to maintain...

Embodiment 2

[0037] A method for fermenting and extracting and purifying L-leucine, comprising the steps of:

[0038] Components of the fermentation medium: glucose 50g / L, (NH 4 ) 2 SO 4 10g / L, corn steep liquor 10g / L, citric acid 0.5g / L, KH 2 PO 4 4g / L, MgSO 4 ·7H 2 O 1.5g / L, MnSO 4 ·H 2 O 10mg / L, FeSO 4 ·7H 2 O 0.1g / L, V B1 5mg / L, V H 0.1mg / L.

[0039] The components of the compound amino acid solution: glutamic acid 10g / L, isoleucine 5 / L, valine 5g / L,

[0040] Components of nutrient solution: glucose 100g / L, glycerol 5g / L, betaine 1g / L;

[0041] Step 1) seed solution (OD) with 8% inoculum 600 value is 9.5) into the fermentation medium, the culture temperature is 37.0°C, the pH of the medium is controlled by adding 20% ​​ammonia water automatically to 7.1, and the dissolved oxygen is controlled at 30% by stirring and ventilation; when the fermentation reaches 12h, the compound Add the amino acid solution into the tank to maintain the concentration of glutamic acid in the t...

Embodiment 3

[0045] Effects of fermentation factors on L-leucine production and cell biomass in fermentation broth.

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Abstract

The invention belongs to the technical field of amino acid production, and discloses a method for fermenting, extracting and purifying L-leucine, which comprises the following steps: step 1) fermentation to produce L-leucine, step 2) sterilization and microfiltration, step 3) Extraction and purification. The method of the invention improves the fermentation yield of leucine, the extraction steps are simple and feasible, and the product has high purity.

Description

technical field [0001] The invention belongs to the technical field of amino acid production, and in particular relates to a method for fermenting, extracting and purifying L-leucine. Background technique [0002] L-leucine ((2R)-2-amino-3,3-dimethylbutanoic acid) chemical name is L-2-amino-4-methylpentanoic acid, white crystal or crystalline powder; odorless, slightly bitter taste . Soluble in formic acid, slightly soluble in water, very slightly soluble in ethanol or ether. The density is 1.038g / cm 3 , melting point greater than 300 ° C. [0003] L-Leucine, L-Isoleucine, and L-Valine are branched-chain amino acids that help promote muscle recovery after training. Among them, L-leucine is the most effective branched-chain amino acid, which can effectively prevent muscle loss because it can be decomposed and converted into glucose faster. [0004] At present, the production method of L-leucine is mainly the fermentation method, and the L-leucine fermentation liquid prod...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/06C07C227/40C07C229/08C12R1/15
CPCC07C227/40C12P13/06C07C229/08
Inventor 包鑫张宗华米永花田辉李江雷韦树高
Owner XINJIANG FUFENG BIOTECH
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