Freeze storage liquid and CAR-T cell freeze storage preparation for human body retransformation

A technology of freezing liquid and cells, which is applied in the field of cell medicine, can solve the problems of long time and inability to inject fresh preparations in time, and achieve good preservation effect and convenient use

Inactive Publication Date: 2019-03-29
SHENZHEN PREGENE BIOPHARMA CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the treatment process, it takes a long time to obtain CAR-T cells from extraction-preparation-expansio

Method used

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  • Freeze storage liquid and CAR-T cell freeze storage preparation for human body retransformation
  • Freeze storage liquid and CAR-T cell freeze storage preparation for human body retransformation
  • Freeze storage liquid and CAR-T cell freeze storage preparation for human body retransformation

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Embodiment Construction

[0013] The technical solutions in the embodiments of the present invention will be clearly and completely described below, obviously, the described embodiments are only some of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0014] In addition, the technical solutions of the various embodiments can be combined with each other, but it must be based on the realization of those skilled in the art. When the combination of technical solutions is contradictory or cannot be realized, it should be considered that the combination of technical solutions does not exist , nor within the scope of protection required by the present invention.

[0015] 1. Freezing solution

[0016] The reagent source that following embodiment adopts:

[0017] Compound Electrolyte ...

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Abstract

The invention discloses freeze storage liquid and a CAR-T cell freeze storage preparation for human body retransformation, wherein the freeze storage liquid is used for freeze storage of CAR-R cells,and is prepared from the following ingredients of 0 to 67.5 (v/v) percent of compound electrolytes injection, 0 to 67.5 (v/v) percent of sodium chloride and dextrose injection, 0 to 20 (v/v) percent of dextran 40 dextrose injection, 20 to 30 (v/v) percent of human serum albumin injection and 5 to 15 (v/v) percent of dimethylsulfoxide, wherein the content of human serum albumin in human serum albumin injection is 20 percent; the compound electrolytes injection contains sodium chloride, sodium gluconate, sodium acetate, potassium chloride and magnesium chloride. By using the technical scheme, the CAR-T cells can be stored for a long period; the use is more convenient and flexible.

Description

technical field [0001] The invention relates to the technical field of cell medicine, in particular to a cryopreservation solution and a CAR-T cell cryopreservation preparation for human reinfusion. Background technique [0002] Chimeric Antigen Receptor T-Cell Immunotherapy, referred to as CAR-T, refers to chimeric antigen receptor T cell immunotherapy. Its basic principle is to use the patient's own immune T cells to eliminate cancer cells. The process mainly includes: 1. From the patient's body Separation of immune T cells; 2. Target modification of T cells through genetic engineering technology and activation of chimeric antigens of T cells at the same time to obtain CAR-T cells; 3. In vitro culture and large-scale expansion of CAR-T cells; 4. The expanded CAR-T cells are infused back into the patient. This method uses cell-targeted therapy and has a significant therapeutic effect on malignant blood tumors (such as acute leukemia and non-Hodgkin's lymphoma). However, d...

Claims

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Application Information

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IPC IPC(8): A01N1/02A61K35/17
CPCA01N1/0221A61K35/17
Inventor 张继帅宋宗培蔡清华郑孟韬苏红昌李华秀包朝乐萌
Owner SHENZHEN PREGENE BIOPHARMA CO LTD
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