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Application of bacteroides fragilis to preparation of medicines for treating and preventing tumors

A technology for Bacteroides fragilis and tumors, applied in the field of biomedicine, can solve the problem of no oncolytic effect, and achieve the effect of inhibiting growth

Inactive Publication Date: 2019-03-29
REVAISSANT SHENZHEN BIOSCIENCES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bifidobacteria are nonpathogenic, obligate anaerobes and have been successfully used to target tumors and as therapeutic vectors, but have not been shown to be oncolytic

Method used

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  • Application of bacteroides fragilis to preparation of medicines for treating and preventing tumors
  • Application of bacteroides fragilis to preparation of medicines for treating and preventing tumors
  • Application of bacteroides fragilis to preparation of medicines for treating and preventing tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 Bacteroides fragilis culture

[0032] training method

[0033] Step 1: Take a freeze-dried strain of Bacteroides fragilis (Bacteroides fragilis), add 200 μL of TSB medium, redissolve, absorb 20 bases, draw a line on the blood plate, and put it in the biochemical incubator after the gas control system of the anaerobic tank is pumped 37 dishes, anaerobic culture for 48 hours;

[0034] Step 2: Pick a monoclonal colony and insert it into 10mL TSB medium, 37, and anaerobic culture for 12h;

[0035] Step 3: Take 1 bottle of 500mL TSB medium, insert 1% (v / v) bacteria, 37, and anaerobic culture for 48h;

[0036] Step 4: Take the bacterial solution and centrifuge at 6000rpm for 10min. Wash twice with normal saline, and finally reconstitute the sludge with normal saline for use and count the viable bacteria.

Embodiment 2

[0037] Embodiment 2 Bacteroides fragilis is to the experiment of the therapeutic effect of mouse tumor (melanoma)

[0038] figure 1 Schematic diagram of the experimental flow for detecting the therapeutic and preventive effects of Bacteroides fragilis and inactivated Bacteroides fragilis on mouse tumors (melanoma).

[0039] 1. Training method

[0040] The culture method of Bacteroides fragilis is the same as in Example 1.

[0041] 2. Sample preparation

[0042] 1) Preparation of live Bacteroides fragilis ZY-312 cells

[0043] Step 1: Take a freeze-dried and preserved strain, add 200 freeze-dried and preserved bacteria culture medium, redissolve, absorb 20 bases, draw a line on the blood plate, and put it in a biochemical incubator at 37 degrees after the gas control system of the anaerobic tank is pumped , Anaerobic culture for 48h;

[0044] Step 2: Pick a single clonal colony and inoculate into 10mL TSB medium, base 37, culture anaerobically for 12h;

[0045] Step 3: Ta...

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Abstract

The invention relates to the technical field of application of bacteroides fragilis and particularly relates to bacteroides fragilis, and an application of a treatment or prevention composition, containing the bacteroides fragilis, for treating and / or preventing tumors. The invention also provides the bacteroides fragilis, as well as an application of preparing a medicine for treating and / or preventing tumors with the bacteroides fragilis, thus significantly inhibiting growth of tumors. The invention also provides applications of the bacteroides fragilis in preparation of medicine compositions, foods, healthcare products and food additives, which are used for treating and / or preventing tumors.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of using Bacteroides fragilis or a composition comprising using Bacteroides fragilis to treat and / or prevent tumors. Background technique [0002] Cancer has become the "first killer" of human beings. The "Global Cancer Report 2014" published by the World Health Organization (WHO) is under the responsibility of the International Cancer Research Center (IARC), the official cancer agency under the WHO, and more than 250 scientists from more than 40 countries participated in the compilation. The overall situation and prevalence trends of 28 cancers in the country were comprehensively described and analyzed. The report predicts that global cancer cases will show a rapid growth trend, from 14 million in 2012 to 19 million in 2025 and 24 million in 2035. The main difficulty in cancer treatment, especially advanced tumor treatment, is the inability to control cancer metastas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/741A61P35/00A23L33/135
CPCA23L33/135A61K35/741A23V2002/00A23V2200/308A61K35/74A61P35/00
Inventor 曾谷城
Owner REVAISSANT SHENZHEN BIOSCIENCES CO LTD
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