A multi-angle non-destructive observation method and device for internal channels of a microfluidic chip

Abstract

The invention discloses a method and a device for multi-angle non-destructive observation of the internal channel of a microfluidic chip. The device comprises: a first part, namely, a split type clamping unit, which is composed of a left-side module and a right-side module; and a second part, namely, a main body container, which is used for mounting the split type clamping unit and holding a liquid. The method and the device provided by the invention aim that a majority of laboratories only with conventional optical microscopes not only can realize planar observation to the morphology of the internal channel of the microfluidic chip from the upper and lower sides of the top part and the bottom part of the internal channel of the microfluidic chip, but also can realize multi-angle observation to the morphology of the internal channel of the microfluidic chip under the premises of no cutting and no destruction of the microfluidic chip. Thus, under the premises of no use of expensive devices like a confocal microscope and destroying of the functions of the microfluidic chip without cutting of the microfluidic chip, the morphology characteristics of the internal channel of the microfluidic chip can be observed through a cut rough plane; multi-angle observation to the internal channel of the microfluidic chip is realized, so a processed planar or non-planar microchannel stereoscopicand comprehensive geometrical morphology image is obtained.

Description

technical field [0001] The invention relates to a multi-angle nondestructive observation method and device for internal channels of a microfluidic chip. The invention belongs to the field of microfluidic technology. Background technique [0002] For a long time, the tiny volume (10 -15 ~10 -9 L) Liquids, namely micro-droplets, play an important role in petrochemical, food additives, pharmaceutical manufacturing, cosmetics preparation and other industrial fields. However, the traditional "top-down" preparation method is difficult to precisely control the composition, size and flux of these microdroplets, which restricts the further development of microdroplet-based engineering applications. [1] . The rapid development of droplet microfluidics technology in recent years provides a new method for continuously and rapidly generating microdroplets with uniform size and controllable composition, mainly based on the interface between the immiscible discrete phase and the contin...

AI Technical Summary

Problems solved by technology

However, due to the cutting step of the PDMS channel in the manufacturing process, the roughness of the cut part of the side wall is very large. Without cutting the microchannel, it is difficult for the conventional optical microscopes in most laboratories to observe through the rough surface. The shape and size of the specific channel can only be observed through the smooth surfaces on the upper and lower sides, and other planes cannot be observed from other angles, which causes great inconvenience in scientific research and production.

Images