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Application of ATXN2L (Ataxin-2like) as marker capable of assisting in evaluating gastric carcinoma oxaliplatin secondary drug resistance

An oxaliplatin and secondary technology, which is applied in the function and application of genes and proteins, and can solve problems affecting the efficacy of chemotherapy, etc.

Active Publication Date: 2019-04-02
NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, oxaliplatin resistance often occurs, affecting the efficacy of chemotherapy

Method used

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  • Application of ATXN2L (Ataxin-2like) as marker capable of assisting in evaluating gastric carcinoma oxaliplatin secondary drug resistance
  • Application of ATXN2L (Ataxin-2like) as marker capable of assisting in evaluating gastric carcinoma oxaliplatin secondary drug resistance
  • Application of ATXN2L (Ataxin-2like) as marker capable of assisting in evaluating gastric carcinoma oxaliplatin secondary drug resistance

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Embodiment Construction

[0016] 1. Experimental materials

[0017] 1. Tissue specimens

[0018] Tissue section: Gastric cancer resection specimens were selected from Nanfang Hospital of Guangzhou Southern Medical University, including radical resection and palliative resection.

[0019] The inclusion criteria for pathological slides were: 1. Gastric cancer was confirmed by pathology; 2. Pathological slides were from the primary tumor of the stomach.

[0020] Exclusion criteria for pathological slides were: 1. Controversial pathological diagnosis; 2. Incomplete clinical treatment.

[0021] According to the patient's intraoperative condition and imaging examination, the patient was classified into TNM staging. According to the above criteria, a total of 48 cases of stage IV and 119 cases of stage I-III gastric cancer tissue samples were included for analysis. The samples were fixed with 10% formalin, embedded in paraffin, and sectioned.

[0022] Fresh gastric cancer specimens: Gastric cancer surgical...

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Abstract

The invention belongs to the field of the function and the application of genes and proteins, and relates to a new application of ATXN2L (Ataxin-2like) as a marker capable of assisting in evaluating gastric carcinoma oxaliplatin secondary drug resistance. Through a clinic specimen, a gastric carcinoma patient with high ATXN2L expression is proved to have bad prognosis. After the ATXN2L is silenced, the sensitivity of a gastric carcinoma cell for the oxaliplatin is enhanced. Through an in vitro induction method, an oxaliplatin drug-resistant strain is obtained, and an MTT (microculture tetrozolium) experiment proves that the oxaliplatin drug-resistant strain resists the oxaliplatin and a malignance degree is higher. Western blot detection finds that the expression of the ATXN2L in the oxaliplatin drug-resistant strain is higher than expression in a sensitive strain. In a flow type detection apoptosis experiment, after the ATXN2L is silenced, the sensitivity of the oxaliplatin drug-resistant strain for the oxaliplatin is increased. Therefore, the ATXN2L can be used as the marker capable of assisting in evaluating gastric carcinoma oxaliplatin secondary drug resistance, and therefore,an existing preparation technology for a kit can be used for providing a kit capable of assisting in evaluating gastric carcinoma oxaliplatin secondary drug resistance.

Description

technical field [0001] The invention belongs to the field of functions and applications of genes and proteins, and relates to a new application of ataxin-2-like protein (ATXN2L), in particular to the application of ATXN2L for preparing drugs for reversing gastric cancer oxaliplatin drug resistance. Background technique [0002] Gastric cancer is a common malignant tumor in my country, and chemotherapy is an important treatment in the comprehensive treatment of gastric cancer. The resistance of tumor cells to chemotherapy drugs limits the efficacy of chemotherapy and often leads to treatment failure in patients. Chemotherapy resistance includes primary drug resistance and secondary drug resistance. Primary drug resistance refers to the tumor’s own resistance to drugs before receiving drug treatment; secondary drug resistance refers to the tumor’s original non-resistant drug resistance. Emerging drug resistance. Solving the problem of primary drug resistance and secondary dr...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 廖旺军林立石敏潘常惬刘彦潭张俊浩
Owner NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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