Pseudomonas strain and application in degrading oxytetracycline thereof

A technology of pseudomonas and oxytetracycline, applied in the above application field, can solve the problems of high cost of treatment methods, complicated procedures and the like, and achieve the effect of reducing adverse effects

Inactive Publication Date: 2019-04-05
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Pseudomonas strain and application in degrading oxytetracycline thereof
  • Pseudomonas strain and application in degrading oxytetracycline thereof
  • Pseudomonas strain and application in degrading oxytetracycline thereof

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[0039] Experimental example

[0040] Inoculate the selected Pseudomonas CGMCC No.13051 into a 250-mL Erlenmeyer flask containing 100 mL of inorganic salt culture solution at a certain inoculum amount, adjust the pH value, and add the oxytetracycline standard to the Erlenmeyer flask. Cultivate in a constant temperature shaker (adjust the temperature and rotation speed) as the experimental group, and use the medium without fungus as the blank control (excluding the influence of the external environment on the concentration of gentamicin).

[0041] Calculate the removal rate according to the removal rate calculation formula:

[0042] Removal rate (%) = (C 0 -C t ) / C 0 ×100

[0043] Where C 0 : The initial concentration of oxytetracycline in the experimental group; C t : Oxytetracycline concentration of the experimental group after the reaction.

[0044] The following is to explore the effects of optimization of microbial degradation conditions, initial concentration of oxytetracycline, ...

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Abstract

The invention discloses a strain of Pseudomonas sp. T4. A preservation number of the strain of Pseudomonas sp. T4 is CGMCC No.13051, the strain of Pseudomonas sp. T4 is already preserved in China general microbiological culture collection center on September 28, 2016, and the address is institute of microbiology, Chinese academy of sciences, No.3, No.1 courtyard, beichen west road, chaoyang district, Beijing. The bacteria can be applied for degrading oxytetracycline in a water body, and a method of the application of the bacteria in degrading the oxytetracycline in the water body comprises thestep that the bacteria are applied into a water body environment to achieve oxytetracycline degradation. By culturing the bacteria and using the bacteria for removing residual oxytetracycline pollutants in the water body, damage of the oxytetracycline for the environment, humanity and animals is reduced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a strain of pseudomonas and its application in degrading oxytetracycline. Background technique [0002] Pharmaceuticals and personal care products (PPCPs), as a new class of pollutants, are attracting increasing attention. According to European Union (EU) statistics, there are about 3,000 PPCPs used in medicine, such as painkillers, Antibiotics etc. Among them, veterinary antibiotics (Veterinary antibiotics, VAs) are frequently used as growth promoters and disease prevention drugs in aquatic and poultry farming, making them continuously enter the environment. [0003] Oxytetracycline (OTC) is one of the common tetracycline antibiotics, a broad-spectrum antibiotic. It is widely used in the world. Since oxytetracycline cannot be well absorbed by animals, about 40%-90% of antibiotics are discharged into the environment and detected in the form of their parent or metabolites. In the U...

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/38C02F101/34
CPCC02F3/34C02F2101/345C02F2101/38C12N1/205C12R2001/38
Inventor 李兆君祁为宁冯瑶成登苗
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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