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A large-batch sample loading device for matrix-assisted laser desorption mass spectrometry

A matrix-assisted laser and mass-batch technology, which is applied in the direction of sample introduction/extraction, discharge tube, particle separation tube, etc., can solve the problems of weakening MALDI analysis speed and prolonging the experiment cycle, so as to shorten the sample analysis time and achieve universal application. High performance and improved experimental efficiency

Active Publication Date: 2020-04-28
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing commercial instruments commonly use 96-well or 384-well plates. If each target is targeted sequentially, the time for loading samples and waiting for crystallization will prolong the experimental period and weaken the advantages of fast MALDI analysis.

Method used

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  • A large-batch sample loading device for matrix-assisted laser desorption mass spectrometry
  • A large-batch sample loading device for matrix-assisted laser desorption mass spectrometry
  • A large-batch sample loading device for matrix-assisted laser desorption mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] combine figure 1 , figure 2 , image 3 , Figure 4 , Figure 5 , Image 6 The bulk sample loading device for matrix-assisted laser desorption mass spectrometry consists of a retractable support 1 , a sample loading channel plate 2 , a sample scraper 3 and a sample pressure plate 4 . The four sides of the telescopic support 1 can be adjusted in length by manual stretching to ensure that it is consistent with the size of the target board used. The four corners of the telescopic support 1 have a four-corner support 5 respectively, and the four-corner support 5 has a flat convex surface. Platform 6 can be adjusted in the vertical direction along with the height adjustment of quadrangular pillar 5. The sample loading channel plate 2 is composed of plane grooves 8 and channels 7 arranged in an array on the plane grooves 8, wherein the array of channels 7 is consistent with the array of target points on the target plate, and penetrates from the surface of the sample load...

Embodiment ２

[0038] In this embodiment, the device described in Example 1 is used. Before loading the sample, the sample loading channel plate 2 is closely attached to the target plate, so that the channel wall and the surface of the target point together form a microcontainer, which can store the sample stably and ensure the subsequent The samples in the channel during operation can be loaded onto the MALDI target plate in equal amounts at the same time.

[0039] In this embodiment, the height of the cylindrical protrusion 9 on the sample platen is 0.1 mm longer than the depth of the channel 7, so that all the samples in the channel 7 can be loaded onto the target point.

[0040] In this embodiment, the channel 7 of the sample loading channel plate 2 is in the shape of a truncated cone;

[0041] In this embodiment, the array of wells 7 of the loading well plate 2 is consistent with the array of target points on the target plate;

[0042] Further, the difference between the axis of the ch...

Embodiment ３

[0044] In this embodiment, using the device described in Example 1, before loading the sample, the sample loading channel plate 2 can also be slightly separated from the target plate, as long as it is ensured that the sample will not drip unevenly through the channel before the sample pressing plate 4 is used. to the target point.

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Abstract

The invention relates to a large-scale sample loading device for matrix-assisted laser desorption mass spectrums. The sample loading device comprises a telescopic support, a sample loading hole channel plate, a sample scraper and a sample pressing plate. The size of the telescopic support can be adjusted to accommodate target plates of a variety of sizes, and the height is adjusted to control thedistance between the sample loading hole channel plate and the target point plates. A hole channel array of the sample loading hole channel plate is consistent with a target array. The sample scraperis used to simply and quickly distribute samples to be tested poured on the sample loading hole channel plate to each hole channel evenly, then the sample pressing plate is used to load the samples inthe hole channels to target points under the effect of external force. According to the device, the large-volume simultaneous loading of the matrix-assisted laser desorption mass spectrums can be achieved, the times of targeting and waiting for crystallization are shortened, and the efficiency of mass spectrum detection is improved.

Description

technical field [0001] The invention relates to the field of analysis and detection instruments, in particular to a large-batch sample loading device for matrix-assisted laser analysis mass spectrometry. Background technique [0002] Matrix-assisted laser desorption ionization (MALDI) is a new mass spectrometric ionization technology developed from laser desorption (LD). The basic principle is that the sample and the matrix are mixed to form a co-crystal on the target point. When the crystal is irradiated with laser light, the matrix molecules can absorb and accumulate energy, and rapidly generate heat, causing the matrix and the sample to be tested to form a gas phase together. The ionization of the sample is completed by the proton transfer of the organic matrix, and finally the ions of the sample to be measured pass through devices such as desolvation and focusing, and arrive at the mass analyzer for analysis. [0003] Matrix-assisted laser desorption mass spectrometry h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): H01J49/04
CPCH01J49/0418H01J49/0463
Inventor 刘颖超张权青李顺祥杨芃原黄元宇
Owner FUDAN UNIV
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