Method for culturing seedlings by using bletilla striata seeds

Abstract

The invention discloses a method for culturing seedlings by using bletilla striata seeds. The method comprises the following steps: (1) spreading the bletilla striata seeds in water, maintaining the environment temperature at 20-25 DEG C, and carrying out illumination culturing until protocorms with leaflets are formed; (2) uniformly sowing the protocorms with the leaflets into a seedling culturecontainer laid with a clean culture medium, and then covering the seedling culture container with a soft and transparent plastic film; and (3) illuminating the protocorms which are sown in the seedling culture container under the light, maintaining the environment temperature at 20-25 DEG C, and carrying out illumination culturing until small pseudobulbs which can be planted is formed. The methodhas the beneficial effects that (1) the seeds germinate in water to form the protocorms, sterile operation and an artificial culture medium are not required, the method is simple and convenient to operate, and the seedling culture cost is reduced; (2) the evenness of grown-up seedlings is high; (3) the growing time of seedlings in the early stage is shortened, and loss caused by infringement of herbivorous pests is reduced; and (4) in a germination stage, an insecticide is not required, the method is environmentally friendly, and pesticide residues are avoided.

Description

technical field [0001] The invention relates to a method for raising seedlings, in particular to a method for raising seedlings by using the seeds of white root, and belongs to the technical field of seedling raising. Background technique [0002] Baiji (Bletilla Striata, also known as Baiji) is a perennial terrestrial herb of the family Orchidaceae, and is one of the commonly used traditional Chinese medicines. Orchid seeds are very small, and it is generally believed that the nutrient stores in orchid seeds are not sufficient to germinate and produce an autotrophic seedling, so symbiosis between orchid seeds and fungi is believed to be necessary. In the past, some researchers have reported symbiotic germination of white and seed, such as: [0003] (1) In 1988, Masuhara and his collaborators isolated 18 strains of fungi from adult white and their symbiotic germination ability was quite different. Although able to form mycelial mass in the embryo, it does not promote germi...

AI Technical Summary

Problems solved by technology

[0006] It can be seen that it is feasible to directly raise seedlings with the seeds of Bletilla chinensis at present, but it is necessary to mix insecticides into the sowing matrix to control pests. Insecticides are used to prevent insects, and the use of insecticides will cause environmental pollution, residual poisons and other hazards

In addition, in the early stage of sowing, it is not only necessary to cover the plastic film to maintain humidity, but also to frequently uncover the film for watering. Uncovering the film can easily lead to the invasion of exogenous pests and damage the newly germinated protocorms, thereby affecting the seedling rate

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