Rice sprout active extract and application thereof in sunscreen cosmetics
A technology of extract and grain bud, which is applied in the active extract of grain bud and its application in sunscreen cosmetics, can solve problems such as difficulties, and achieve the effect of improving purity and dissolution rate
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[0026] The preparation method of this grain bud active extract is as follows:
[0027] Step S1: mixing and incubating the grain bud powder with a cell wall degrading enzyme to obtain an enzymatic hydrolyzate;
[0028] Among them, cell wall degrading enzymes include cellulase and ligninase. Preferably, the mass ratio of cellulase and ligninase is 2-4:1, or the mass ratio is 3:1.
[0029] Further, the temperature for mixing and incubating the above-mentioned grain bud powder and the cell wall degrading enzyme is 30-40°C, or the temperature is 32-38°C, or 34-36°C; the time for mixing and incubating the grain bud powder and the cell wall degrading enzyme is 7- 10 h, or 8-9 h.
[0030] Step S2: Mix the enzymatic hydrolyzate with ethanol solution, heat and reflux for extraction, dissolve the obtained extract in water, and then extract with chloroform, ethyl acetate and ethanol in sequence to obtain an ethyl acetate extract;
[0031] Further, the concentration of the ethanol solut...
Embodiment 1
[0040] The present embodiment provides a kind of gluten bud active extract, and it is prepared by following method:
[0041] a. Mix the grain bud powder with cellulase and ligninase, incubate at 37°C for 8 hours to obtain an enzymatic hydrolyzate, and concentrate under reduced pressure to obtain an enzymatic hydrolyzate;
[0042] b. Mix the enzymatic hydrolyzate with 80% ethanol solution, heat and reflux extraction at 75°C for 2 times, each time for 2h. Dissolving the obtained extract in water, followed by extraction with chloroform, ethyl acetate and ethanol, filtering the obtained ethyl acetate extract with a filter membrane with a pore size of 0.22 μm, and concentrating under reduced pressure to obtain the ethyl acetate extract;
[0043] c. The ethyl acetate extract is loaded into the reverse phase silica gel chromatographic column, wherein the consumption of chromatographic filler is 24 times of the ethyl acetate extract, and the volume concentration is 10%, 20%, 40%, 70% ...
Embodiment 2
[0045] The present embodiment provides a kind of gluten bud active extract, and it is prepared by following method:
[0046] a. Mix the grain bud powder with cellulase and ligninase, incubate at 40°C for 7 hours to obtain an enzymatic hydrolyzate, and concentrate under reduced pressure to obtain an enzymatic hydrolyzate;
[0047] b. Mix the enzymatic hydrolyzate with 90% ethanol solution, heat and reflux extraction at 70°C for 2 times, each time for 3 hours. After dissolving the obtained extract in water, extracting with chloroform, ethyl acetate and ethanol in sequence, filtering the obtained ethyl acetate extract with a filter membrane with a pore size of 0.2 μm, and concentrating under reduced pressure to obtain the ethyl acetate extract;
[0048] c. The ethyl acetate extract is loaded into the reverse phase silica gel chromatographic column, wherein the consumption of the chromatographic filler is 22 times of the ethyl acetate extract, and the concentration by volume is 5%...
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Abstract
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