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Echinococcus granulosus hyperimmune yolk antibody, test strip, preparation method and application

A technology of Echinococcus granulosus, egg yolk antibody, applied to Echinococcus granulosus hyperimmune egg yolk antibody and test strip and preparation, application in reagent or reagent strip or medicine, application in kit, Echinococcus granulosa Ball tapeworm hyperimmune yolk antibody and its preparation field

Pending Publication Date: 2019-04-12
THE FIRST TEACHING HOSPITAL OF XINJIANG MEDICAL UNIVERCITY +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports on the use of egg yolk immunoglobulin (yolk antibody) in the diagnosis of echinococcosis

Method used

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  • Echinococcus granulosus hyperimmune yolk antibody, test strip, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1: A kind of Echinococcus granulosus hyperimmune egg yolk antibody is carried out according to the following preparation method: the first step, the preparation of Echinococcus antigenic protein: adopt 0.9% sterilized normal saline or concentration is 0.01mol / L, pH Wash the Echinococcus worm body with sterilized PBS with a value of 7.2 to 7.4, freeze and thaw the washed Echinococcus worm body repeatedly in liquid nitrogen and 37°C for 3 to 5 times, and freeze and thaw the Echinococcus worm body Crush the body, add 50 μL of protein lysate to every 1 mg of the crushed Echinococcus worm body, and then lyse the lysate to obtain the lysate, centrifuge the lysate, take the supernatant after centrifugation, filter the supernatant to sterilize, and sterilize the lysate The protein concentration in the supernatant was adjusted to 1 mg / mL to obtain the Echinococcus antigenic protein;

[0054] The second step, the preparation of the immunogen: the Echinococcus antigenic...

Embodiment 2

[0067] Example 2: Application of a hyperimmune yolk antibody against Echinococcus granulosus in the preparation of reagents or reagent strips or medicines for diagnosing echinococcosis.

Embodiment 3

[0068] Embodiment 3: A kind of freeze-dried hyperimmune egg yolk antibody is carried out according to the following preparation method:

[0069] Step 1, wash the magnetic beads with MEST buffer for 3 times, then add 1ml to 2ml of magnetic beads into 1ml to 2ml of activator and shake to mix evenly. The activator includes 10mg / mL 1-(3-dimethylaminopropyl)- 3-Ethylcarbodiimide hydrochloride solution and 10 mg / mL N-hydroxysuccinimide solution, and then react at room temperature for 15 min to 1 h in the dark at a rotational speed of 150 r / min. Stand still for 5min to 10min, separate the magnetic beads after the reaction with a magnetic stand to obtain activated magnetic beads, discard the supernatant of the reaction solution, and keep the lower layer of the reaction solution.

[0070] Step 2, dissolving the lower layer of the reaction solution with 0.01mol / L PBST buffer solution with a pH value of 5.0 to 7.2 to obtain a lower layer solution, washing the activated magnetic beads at ...

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Abstract

The invention relates to the technical fields of a yolk antibody, a preparation method thereof and an application thereof, and discloses an echinococcus granulosus hyperimmune yolk antibody, a test strip, a preparation method and application. The echinococcus granulosus hyperimmune yolk antibody is obtained according to the following method: preparation of echinococcus granulosus antigen protein,preparation of immunogen, feeding and immunization of laying hens, and preparation of echinococcus granulosus hyperimmune yolk antibody. The present invention discloses for the first time an echinococcus granulosus hyperimmune yolk antibody, a freeze-dried hyperimmune yolk antibody and the use thereof in the diagnosis of echinococcosis, and when a colloidal gold chromatographic test strip made ofthe echinococcus granulosus hyperimmune yolk antibody and the freeze-dried hyperimmune yolk antibody of the present invention is used to diagnose echinococcosis, the colloidal gold chromatographic test strip has good sensitivity, specificity, accuracy and stability, and a simpler detection method for diagnosis of echinococcosis with lower cost is provided.

Description

technical field [0001] The invention relates to the technical field of egg yolk antibody and its preparation method and application, and relates to a kind of Echinococcus granulosus hyperimmune yolk antibody, a test strip, a preparation method and application thereof, which include a Echinococcus granulosus hyperimmune yolk antibody and Its preparation method and its application in the preparation of reagents or reagent strips or medicines for diagnosing echinococcosis; it also includes a freeze-dried hyperimmune egg yolk antibody and its preparation method and its use in the preparation of reagents or reagent strips for diagnosing echinococcosis or Application in medicine; also includes a colloidal gold chromatography test paper strip, its preparation method and its application in the preparation of a test kit for diagnosing echinococcosis. The test strip in the title of the invention represents a colloidal gold chromatography test strip. Background technique [0002] Hyda...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/531G01N33/558G01N33/543
CPCG01N33/531G01N33/54326G01N33/558G01N33/6854G01N2333/43543
Inventor 巩月红温浩王建华高晓黎段新宇滕亮赵军高惠静杨剑锐孙其何丽平
Owner THE FIRST TEACHING HOSPITAL OF XINJIANG MEDICAL UNIVERCITY
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