Method for synchronously detecting seventeen antipsychotics in blood sample

A technology for antipsychotic drugs and blood samples, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of simultaneous detection of antipsychotic drugs, and achieve the effect of avoiding distortion of test results and simple sample processing

Inactive Publication Date: 2019-04-23
JINAN YING SHENG BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the existing method, only a small number of antipsychotic drugs can be detected simultaneously, and it is impossible to detect more than 15 antipsycho...

Method used

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  • Method for synchronously detecting seventeen antipsychotics in blood sample
  • Method for synchronously detecting seventeen antipsychotics in blood sample
  • Method for synchronously detecting seventeen antipsychotics in blood sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0064] (1) Prepare the sample to be tested

[0065] Add methanol:acetonitrile (volume ratio 1:1) solution to the serum sample, vortex and centrifuge, take the supernatant into a sample bottle, blow dry with nitrogen, and redissolve the sample in 70% v / v methanol aqueous solution , 0.2 μm filter membrane to obtain the sample to be tested.

[0066] Wherein, the volume ratio of serum, methanol, and acetonitrile is 2:1:1, and the volume ratio of serum to 70% methanol aqueous solution is 2:1.

[0067] (2) Ultra-high performance liquid chromatography-mass spectrometry detection

[0068] The sample to be tested enters the chromatographic column for separation through the gradient elution mode, and the reference conditions of liquid chromatography are:

[0069] Chromatographic column: C18 column (Waters ACQUITY HPLC BEH C18 100mm×2.1mm, 1.7μm)

[0070] Column temperature: 40°C

[0071] Injection volume: 20μL

[0072] Flow rate: 250μL / min

[0073] Mobile phase A: HPLC grade water...

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Abstract

The invention discloses a method for synchronously detecting seventeen antipsychotics in a blood sample. The method comprises the steps that the sample is extracted with a mixed solution of methanol and acetonitrile to obtain supernate, and after a solvent is removed from the supernate, the supernate is dissolved in a methanol aqueous solution and filtered to obtain a specimen; a high performanceliquid chromatography tandem mass spectrometry method is adopted to detect the specimen; the sample is serum or plasma, and the antipsychotics are amisulpride, aripiprazole, dehydro aripiprazole, chlorpromazine, clozapine, desmethylclozapine, fluphenazine, haloperidol, olanzapine, paliperidone, perphenazine, quetiapine, risperidone, 9-hydroxyrisperidone, sulpiride, ziprasidone and thioridazine; inthe high performance liquid chromatography, a mobile phase A is an aqueous solution of formic acid, and a mobile phase B is a methanol solution of the formic acid; and the mass spectrometry adopts amulti-ion reaction monitoring mode of positive ion electrospray ionization.

Description

technical field [0001] The invention belongs to the detection field of blood drug concentration, and in particular relates to a method for simultaneously detecting 17 kinds of antipsychotic drugs in a blood sample. Background technique [0002] Since the discovery of the antipsychotic effect of chlorpromazine in psychiatry, about 130 drugs have been developed in succession in the past 60 years. These drugs have become effective and essential in the treatment of mental disorders and psychiatric symptoms. Although great medical value and economic benefits have been achieved, the therapeutic effect on many patients is far from satisfactory. The blockade of various neurotransmitters in the central nervous system by psychotherapeutic drugs is the biochemical basis for their antipsychotic effects, but it is followed by a variety of related toxic and side effects on patients, including sexual dysfunction, high prolactin Hyperemia, tardive dyskinesia, acute extrapyramidal reaction...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 冯振景叶松弭兆元
Owner JINAN YING SHENG BIOTECH
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