Lipid type antibacterial composition, extraction method, detection method and application
The technology of an antibacterial composition and an extraction method, applied in the biological field, can solve problems such as difficulty in obtaining an antibacterial effect, and achieve the effects of good extraction effect and high extraction rate
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Embodiment 1
[0030] The separation and preparation steps of the 3rd instar black soldier fly larva lipid antibacterial extract are as follows:
[0031] (1) Empty the intestinal contents of black soldier fly larvae
[0032] The 3rd instar black soldier fly larvae were placed in the breeding box, and they were not fed overnight until the intestinal contents of the larvae were cleared.
[0033] (2) Cleaning and disinfection
[0034] Put the black soldier fly larvae that had been fasting overnight into a conical flask, wash it three times with distilled water, filter out the water, sterilize it once with 75% ethanol, and filter it out for later use.
[0035] (3) Removal of larval gut
[0036] The spare larvae were filtered and placed on an ultra-clean bench (SW-CJ-IF), operated aseptically, carefully incised with a scalpel along the midline of the larvae's abdomen, and the larvae intestines were taken out with sterile tweezers. The parasites and intestines were stored in clean containers at...
Embodiment 2
[0044] The separation and preparation steps of the lipid antibacterial extract of 3.5 instar black soldier fly larvae are as follows:
[0045] (1) Empty the intestinal contents of black soldier fly larvae
[0046] The 3.5-instar black soldier fly larvae were placed in the breeding box, and were not fed overnight until the intestinal contents of the larvae were cleared.
[0047] (2) Cleaning and disinfection
[0048] Put the black soldier fly larvae that had been fasting overnight into a conical flask, wash it three times with distilled water, filter out the water, sterilize it once with 75% ethanol, and filter it out for later use.
[0049](3) Removal of larval gut
[0050] The spare larvae were filtered and placed on an ultra-clean bench (SW-CJ-IF), operated aseptically, carefully incised with a scalpel along the midline of the larvae's abdomen, and the larvae intestines were taken out with sterile tweezers. The parasites and intestines were stored in clean containers at l...
Embodiment 3
[0056] The separation and preparation steps of the 4th instar black soldier fly larva lipid antibacterial extract are as follows:
[0057] (1) Empty the intestinal contents of black soldier fly larvae
[0058] The 4th instar black soldier fly larvae were placed in the breeding box, and they were not fed overnight until the intestinal contents of the larvae were cleared.
[0059] (2) Cleaning and disinfection
[0060] Put the black soldier fly larvae that had been fasting overnight into a conical flask, wash it three times with distilled water, filter out the water, sterilize it once with 75% ethanol, and filter it out for later use.
[0061] (3) Removal of larval gut
[0062] The spare larvae were filtered and placed on an ultra-clean bench (SW-CJ-IF), operated aseptically, carefully incised with a scalpel along the midline of the larvae's abdomen, and the larvae intestines were taken out with sterile tweezers. The parasites and intestines were stored in clean containers at...
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