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Preparation method and application of Mo3S4-coated gold nano-rod

A technology of molybdenum tetrasulfide and gold nanorods is applied in the fields of chemical biosensing and biological detection, which can solve problems such as human poisoning, and achieve the effects of simple operation, simple method and high detection sensitivity.

Active Publication Date: 2019-04-26
HUNAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once these substances enter the human body, they will cause poisoning and there is no specific drug treatment for animal zearalenone poisoning

Method used

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  • Preparation method and application of Mo3S4-coated gold nano-rod
  • Preparation method and application of Mo3S4-coated gold nano-rod
  • Preparation method and application of Mo3S4-coated gold nano-rod

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Preparation of gold nanorods coated with molybdenum tetrasulfide

[0034] Take 5mL gold nanorods, first add 0.1mmol / L Na 2 S 2 o 3 20μL, then add 0.1mmol / L Mocl 540μL, reacted for 6 hours, centrifuged at 10000r / min for 10min, and dispersed with secondary water to obtain gold nanorods coated with molybdenum tetrasulfide, whose shape is as follows figure 1 As shown, the morphology of the gold nanorods is completely preserved and a shell-like structure is formed on the surface of the gold rods, and the longitudinal plasmon absorption peak of the composite is blue-shifted compared with the longitudinal plasmon absorption peak of the gold nanorods, as shown in figure 2 As shown, this is because the refractive index of the surface of the gold rod changes due to the interaction between molybdenum tetrasulfide and the gold rod. image 3 The powder crystal diffraction results show that molybdenum tetrasulfide is coated on the surface of gold nanorods in the form of crystal...

Embodiment 2

[0036] Molybdenum tetrasulfide-coated gold nanorods mimic enzyme properties of peroxide for the detection of zearalenone:

[0037] First, add 300 μL of molybdenum tetrasulfide-coated gold nanorods to the centrifuge tube, then add 500 μL of 1.0×10 -13 The DNA aptamer ZEN-aptamer of μmol / L zearalenone, connected with ZEN-aptamer, washed three times with 0.01mol / L PBS, then added different concentrations of zearalenone solutions, reacted for 20 minutes, and used After washing with double distilled water three times, add a hydrogen peroxide solution with a concentration of 50mmol / L to react for 30 minutes, and then add a TMB solution with a concentration of 5mmol / L. After 30 minutes, its color changes as Image 6 As shown in the figure, 0 and 10 are added from left to right in the figure -7 、10 -6 、10 -5 、10 -4 、10 -3 、10 -2 For zearalenone in mmol / L, it can be seen from the figure that the higher the concentration of zearalenone, the lighter the color of TMB. According to...

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Abstract

The invention discloses a preparation method and application of a Mo3S4-coated gold nano-rod. The preparation method of the Mo3S4-coated gold nano-rod comprises the steps that gold nano-rods react with a sodium thiosulfate solution and a molybdenum pentachloride solution for 4-8 h, and then the Mo3S4-coated gold nano-rods with peroxide mimetic enzyme activity is obtained. The obtained Mo3S4-coatedgold nano-rod mimetic enzyme is used for detecting the content of fungaltoxin zearalenone. The feature of hydrogen peroxide catalytic decomposition of the Mo3S4-coated gold nano-rods is utilized, according to specific binding between an antigen and an antibody, the specificity effect is conducted between zearalenone and the zearalenone aptamer modified by the Mo3S4-coated gold nano-rods, and thenthe purpose of detecting the zearalenone is achieved. The Mo3S4-coated gold nano-rods are utilized to detect the zearalenone, which is high in flexibility, obvious in phenomenon and easy and convenient to operate, and the concentrate of the zearalenone in crop can be effectively and accurately detected.

Description

technical field [0001] The invention belongs to the technical field of chemical biosensing and biological detection, and in particular relates to a preparation method and application of gold nanorods coated with molybdenum tetrasulfide. Background technique [0002] Enzymes are indispensable to life activities. Many reactions in the human body require the catalysis of enzymes. It can reduce the activation energy of chemical reactions, speed up the rate of chemical reactions, and control the degree of chemical reactions. Natural enzymes are proteins, nucleic acids or their complexes that are produced in vivo and have properties such as high efficiency, specificity, diversity, and ability to react specifically with substrates. They are widely used in food, industry, agriculture, and medicine. Wide range of applications. However, natural enzymes also have many shortcomings. For example, the sensitivity of external environmental conditions (strong acid, strong alkali, high tem...

Claims

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Application Information

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IPC IPC(8): B22F1/02B22F9/24G01N21/31B82Y40/00
CPCG01N21/31B82Y40/00B22F9/24B22F1/16B22F1/054
Inventor 黄昊文花欣怡汪志芳阳秀梅
Owner HUNAN UNIV OF SCI & TECH
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