Rapid detection method for rimonabant

A detection method, the technology of rimonabant, applied in the field of analytical chemistry detection, can solve the problems of high requirements for operators, cumbersome process, high detection cost, etc., and achieve the effect of improving extraction efficiency, increasing solubility and good specificity

Active Publication Date: 2016-05-04
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The currently reported detection methods for rimonabant are mainly high performance liquid chromatography and high performance liquid chromatography-mass spectrometry, which have problems such as long detection period, cumbersome process, h

Method used

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  • Rapid detection method for rimonabant
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  • Rapid detection method for rimonabant

Examples

Experimental program
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Example Embodiment

[0031] Example 1: Detection of tea

[0032] Reagent preparation: alkalizing solution is 0.5mol / L sodium hydroxide-water solution; extracting solution is petroleum ether (60-90); acidifying solution is 1mol / L hydrochloric acid aqueous solution; compound solution is ethanol; detection solution is bismuth potassium iodide test Solution (take 0.85g of bismuth subnitrate, add 10ml of glacial acetic acid and 40ml of water to dissolve, add 20ml of 4% potassium iodide aqueous solution, shake well, and it is ready.)

[0033] Sample: Slimming tea (the tea, the labelled dosage is 1 bag, and the LC-MS / MS method determines that this product contains Rimonabant.)

[0034] The detection method, the steps are:

[0035] 1) Take 1 bag of the above sample to be tested, grind the contents finely, take 1g of powder, add 3mL of 0.5mol / L sodium hydroxide-water solution, shake for 3min, add 3mL of petroleum ether (60-90), shake for 4min , Let stand for 4 minutes, and take the upper layer solution.

[0036] 2...

Example Embodiment

[0039] Example 2: Detection of capsules

[0040] Reagent preparation: alkalizing solution is 0.4mol / L potassium hydroxide-aqueous solution; extracting solution is cyclohexane; acidifying solution is 0.9mol / L phosphoric acid aqueous solution; compound solution is methanol; detection solution is potassium bismuth iodide test solution (take times 0.85g of bismuth nitrate, add 10ml of glacial acetic acid and 40ml of water to dissolve, add 20ml of 4% potassium iodide aqueous solution, shake well, and it is ready.)

[0041] Sample to be tested: Capsules (capsules, the labeled dosage is 2 capsules, and the LC-MS / MS method determines that this product contains Rimonabant.)

[0042] The detection method, the steps are:

[0043] The detection method, the steps are:

[0044] 1) Take 5 pieces of the above-mentioned sample to be tested, and grind the contents into fine pieces. Take 0.5g of powder, add 1.5mL of 0.4mol / L potassium hydroxide-water solution, shake for 2min, add 1.5mL of cyclohexane, sh...

Example Embodiment

[0048] Example 3: Detection of tablets

[0049] Reagent preparation: alkalizing solution is 0.6mol / L sodium hydroxide-aqueous solution; extracting solution is n-hexane; acidifying solution is 1.1mol / L sulfuric acid aqueous solution; compound solution is ethanol; test solution is bismuth potassium iodide test solution (take hyponitric acid 0.85g of bismuth, add 10ml of glacial acetic acid and 40ml of water to dissolve, add 20ml of 4% potassium iodide aqueous solution, shake well, and get it.)

[0050] Sample: Slimming effervescent tablet (tablet, the indicated dosage is 1 tablet, the product does not contain rimonabant as determined by the LC-MS / MS method.)

[0051] The detection method, the steps are:

[0052] 1) Take 1g of the powder to be tested above, add 3mL of 0.6mol / L sodium hydroxide-water solution, shake for 4min, add 3mL of n-hexane, shake for 5min, stand for 5min, and take the upper layer solution.

[0053] 2) Add 3mL 1.1mol / L sulfuric acid aqueous solution to step 1) upper l...

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Abstract

The invention discloses a rapid detection method for rimonabant. As to-be-detected samples undergo three steps, i.e., alkalization and dispersion, extraction and separation, and acidification and precipitatioin, whether the samples contain rimonabant can be preliminarily determined according to results of a precipitation reaction; and suspected positive samples are further subjected to an alkaloid precipitation reaction so as to confirm whether the samples contain rimonabant or not. The method utilizes different dissolvability of different substances to remove interferent in three steps and more accurately determines whether the samples contain rimonabant or not by using two precipitation reactions of different mechanisms. The detection method provided by the invention is simple to operate, low in analysis cost, strong in specificity, high in sensitivity and applicable to on-site rapid detection of illegal addition of rimonabant in fat-reducing Chinese patent medicines, health food and foodstuffs.

Description

technical field [0001] The invention belongs to the field of analytical chemistry detection and relates to a rapid detection method for rimonabant. Background technique [0002] In recent years, with the improvement of people's living standards, the obesity problem caused by factors such as unreasonable diet structure and lack of exercise has become increasingly serious. According to the World Health Organization, 39% of adults aged 18 and over were overweight and 13% were obese in 2014. Obesity can induce or aggravate diabetes, fatty liver, cardiovascular and cerebrovascular diseases, sleep apnea syndrome, musculoskeletal disorders, and certain cancers, and has become one of the ten major threats to human health. Along with people's urgent need for weight loss, various weight loss products are also increasing day by day, and good and bad people are mixed. Rapid weight loss with chemical drugs usually brings obvious adverse reactions, while Chinese patent medicines, health...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 咸瑞卿梁翠荣石峰王杰巩丽萍郭常川邢晟姜树银
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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