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Down's Syndrome Therapeutic Composition and Its Application

A technology of Down's syndrome, therapeutic composition, applied to TGF-β pathway protein and Wnt signal inhibitor, application of the composition for the treatment of Down's syndrome, the field of Down's syndrome therapeutic composition, capable of solving Down's syndrome Syndrome treatment drugs and methods have no obvious effect, etc., to achieve the effect of easy promotion and use, good effect and strong performance

Active Publication Date: 2021-02-09
THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problem that the effect of the medicine and method for treating Down's syndrome in the prior art is not obvious, and proposes a composition for treating Down's syndrome and its application

Method used

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  • Down's Syndrome Therapeutic Composition and Its Application
  • Down's Syndrome Therapeutic Composition and Its Application
  • Down's Syndrome Therapeutic Composition and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Such as figure 1 Shown is a wild-type normal embryo of a live zebrafish embryo fertilized for 24 hours, as indicated by the arrow.

[0035] In order to better verify the therapeutic effects of TGF-β pathway proteins and Wnt signaling inhibitors on Down syndrome, a Down syndrome model was first established. Such as figure 2 As shown, the Down syndrome model can well simulate the abnormalities and treatment conditions of Down syndrome, such as figure 2 Where the arrow points, the embryo is severely deformed. If the Down syndrome model behaves like image 3 Shown, on behalf of Down syndrome treatment has a certain effect.

[0036] The specific process is as follows:

[0037] By injecting 50 pg DYRK1A mRNA into zebrafish freshly fertilized embryos.

[0038] At present, we add TGF-β pathway protein TGF-β1 and Wnt signaling inhibitor to the culture medium of cultured embryos, the concentration of TGF-β pathway protein is 8-10ng / ml, and the concentration of Wnt signali...

Embodiment 2

[0042] Such as figure 1 Shown is a wild-type normal embryo of a live zebrafish embryo fertilized for 24 hours, as indicated by the arrow.

[0043] In order to better verify the therapeutic effects of TGF-β pathway proteins and Wnt signaling inhibitors on Down syndrome, a Down syndrome model was first established. Such as figure 2 As shown, the Down syndrome model can well simulate the abnormalities and treatment conditions of Down syndrome, such as figure 2 Where the arrow points, the embryo is severely deformed. Then use TGF-β pathway protein + Wnt signaling inhibitor. If the Down syndrome model behaves like image 3 Shown, on behalf of Down syndrome treatment has a certain effect.

[0044] The specific process is as follows:

[0045]In vitro, DYRK1A was first cloned behind the heat-activated promoter hsp70 to construct the hsp70:DYRK1A plasmid, and then the constructed plasmid and tol2 transposase were injected into zebrafish freshly fertilized embryos, and heat-acti...

Embodiment 3

[0050] Such as Figure 4 Shown is the heart site of a wild-type normal embryo fertilized 48 hours from zebrafish, as indicated by the arrow.

[0051] In order to better verify the therapeutic effects of TGF-β pathway proteins and Wnt signaling inhibitors on Down syndrome, a Down syndrome model was first established. Such as Figure 5 As shown, the Down syndrome model can well simulate the abnormalities and treatment conditions of Down syndrome, such as Figure 5 Where the arrow points, the heart has abnormalities similar to Down's syndrome. Then use TGF-β pathway protein + Wnt signaling inhibitor. If the Down syndrome model behaves like Figure 6 Shown, on behalf of Down syndrome treatment has a certain effect.

[0052] The specific process is as follows:

[0053] By injecting 50 pg DYRK1A mRNA into zebrafish freshly fertilized embryos.

[0054] At present, we add TGF-β pathway protein TGF-β1 and Wnt signaling inhibitor to the culture medium of cultured embryos, the con...

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Abstract

The invention discloses a composition for treating Down's syndrome and its application, belonging to the field of biotechnology. This application aims at Down's syndrome, and proposes the application of TGF-β pathway protein and Wnt signaling inhibitor in the preparation of a composition for treating Down's syndrome, and proposes a composition for treating Down's syndrome, which includes TGF ‑β Pathway Proteins and Wnt Signaling Inhibitors. The composition has an obvious therapeutic effect on the Down's syndrome model, and the reagents included in the composition are all small molecular weight proteins or drugs, have good effects on embryos, and are convenient for popularization and use.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to a composition for treating Down's syndrome and its application, and specifically discloses a composition for treating Down's syndrome, as well as TGF-β pathway protein and Wnt signal inhibitors used in the preparation of Down's syndrome. Syndrome composition on the application. Background technique [0002] Down syndrome (Down’s syndrome, DS), also known as trisomy 21 and congenital stupidity, is the most common autosomal abnormality. The incidence rate is 1 / 300 in pregnant women over 35 years old, and 1 / 800 in pregnant women under 35 years old. Most hospitals have carried out experiments on the serological screening of Down syndrome in pregnant women. For pregnant women who screen positive, amniotic fluid cell culture is routinely used for karyotype analysis to confirm the diagnosis. However, cell culture requires high conditions, the method is cumbersome, and takes a long time (10-14 d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/06A61K38/18A61K31/09A61P25/28
Inventor 孙华钦刘燕燕林子媛
Owner THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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