Method for identifying origin of atractylodes based on stable isotope ratios

Abstract

The invention discloses a method for identifying the origin of atractylodes based on stable isotope ratios and particularly relates to the origin identification field. The method comprises steps thatS01, multiple atractylodes samples of different real origins are selected; S02, a stable isotope ratio A of each of the atractylodes samples is measured, a stable isotope ratio database of the atractylodes samples from the real origins is constructed; S03, according to the stable isotope ratio database of the atractylodes samples from the real origins, the statistical software is utilized to perform orthogonal partial least squares identification analysis, and a traceability model for the origin is established; S04, an to-be-tested atractylodes sample of the unknown real origin is selected, and a stable isotope ratio B of the atractylodes sample from the unknown origin is determined; and S05, the stable isotope ratio B obtained above is inputted as an input value to the origin traceabilitymodel, and the corresponding origin discrimination result is obtained and outputted. The method has advantages of high identification accuracy, small quantity of detection indexes, simple sample pre-processing and small amount of samples.

Description

technical field [0001] The invention relates to origin identification, in particular to a method for identifying the origin of Atractylodes Rhizoma based on stable isotope ratio. Background technique [0002] Atractylodes macrocephala (Atractylodes macrocephala Koidz.) is the dry rhizome of the Compositae plant. It is one of the famous "Zhe Bawei" in traditional Chinese medicine. , there is a saying that "the north is used to the south". Atractylodes is mainly produced in Zhejiang, Anhui, Hunan, Hubei and other provinces, Jiangxi, Fujian, Sichuan and other provinces are also cultivated. There are more than 200 counties and cities nationwide that cultivate Atractylodes, but the quality varies. Since the Song Dynasty, Zhejiang Yuqian, Xinchang, Pan'an and other places have gradually developed into Atractylodes Dao production areas due to the high quality of Atractylodes produced. Since authentic medicinal materials have been regarded as high-quality medicinal materials since...

AI Technical Summary

Problems solved by technology

[0003] The identification of the origin of Atractylodes macrocephala has always been a big problem. The traditional manual identification method needs to rely on the personal experience of experts and cannot be popularized on a large scale. Currently, the commonly used molecular biology methods and chemical composition analysis methods (such as: DNA barcode technology, high performance liquid chromatography fingerprints, gas chromatography fingerprints, infrared spectrum fingerprints) have their own limitations

Among them, molecular biology methods can accurately distinguish the small differences between different germplasms of a specific Chinese medicinal material, but they cannot be distinguished if the provenance is the same. Molecular marker technology will be powerless in this case

The accuracy of the chemical composition analysis method is not high enough. Existing reports show that there are sometimes differences in some chemical components of Atractylodes macrocephala produced in different regions, but it is impossible to directly infer the origin of Atractylodes macrocephala based on these data, and the chemical components are easily affected by factors other than the place of origin. Influence of factors (eg provenance)

Images