Washing-free kelp double-layer makeup removing lotion and preparing method thereof
A technology of macroalgae and solution, applied in the field of cosmetic compositions, can solve the problems of strong irritation and unsuitable for makeup removal and the like
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Embodiment 1
[0085] The preparation method of embodiment 1 macroalgae ferment
[0086] (1) Dry the macroalgae and beat it into macroalgae powder, add distilled water to mix, and then ultrasonically disperse and dissolve. Use a 0.22μm membrane filter to remove fat;
[0087] (2) continuously stirring the obtained macroalgae solution, and adding 1-5% NaOH solution by mass percentage therein to adjust its pH value;
[0088] (3) After stirring evenly, sterilize in an autoclave at 121° C. for 15 minutes, and then cool to 25° C. to obtain a macroalgae mixture;
[0089] (4) The culture solution is composed of the following components by weight percentage: 0.2% beef extract, 0.8% peptone, 0.5% sodium chloride, 2% agar, and 0.8% glucose. Adjust its pH value to 6.5-7.5 with 2% NaOH solution by mass percentage, then mix Bacillus subtilis, Bacillus licheniformis and Aspergillus niger in a certain ratio to form a mixed bacterium, and inoculate it in the culture solution;
[0090] (5) adding the nutri...
Embodiment 2
[0097] Example 2 Evaluation of free radical scavenging ability (in vitro DPPH method)
[0098] Accurately weigh 3.5mg of DPPH reagent, dissolve it with absolute ethanol, and quantitatively transfer it to a 10mL volumetric flask, dilute to the mark with absolute ethanol, take 2mL into a 100mL volumetric flask, and shake well to obtain a concentration of 0.0178mmol / L DPPH The solution was stored in the refrigerator for later use.
[0099] Accurately weigh 2 mg each of the dried macroalgae enzymes prepared by methods 1-11, dissolve them in absolute ethanol, and quantitatively transfer them to a 50mL volumetric flask, use absolute ethanol to measure to the mark, take 10mL into a 100mL volumetric flask, and shake well The obtained concentration is 4mg / L macroalgae enzyme test solution.
[0100] Add 4.0mL DPPH solution and 4.0mL macroalgae enzyme test solution in sequence to a 10mL colorimetric tube, then add absolute ethanol to the mark, mix well and immediately use a 1cm colorime...
Embodiment 3
[0104] Embodiment 3 Preparation of Macroalgae Oil Extract
[0105] (1) After drying the macroalgae, beat it into macroalgae powder, add distilled water to mix, and perform ultrasonic treatment in an ice bath;
[0106] (2) mixing the macroalgae solution obtained in step (1) with squalane, and extracting by shaking at a certain temperature;
[0107] (3) After the extraction is finished, add β-cyclodextrin in proportion to the obtained macroalgae mixture, and refine it by shaking at a certain temperature;
[0108] (4) Stand still for 2 hours after shaking, and perform centrifugation at a speed of 8000 r / min for 10 minutes.
[0109] (5) Filter the upper oil phase twice with a filter membrane with a specification of 0.22 μm to obtain macroalgae oil extract.
[0110] Table 3 The parameters of each preparation method of macroalgae oil extract
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