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Preparation method of one-dimensional magnetic nanowires for biochemical analysis

A magnetic nanometer and biochemical analysis technology, applied in the field of preparation of one-dimensional magnetic nanowires, can solve the problems of long reaction time and complicated preparation process of magnetic microspheres, and achieve the effect of enhancing detection sensitivity

Active Publication Date: 2019-05-10
珠海启尼亚生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the detection sensitivity of the magnetic microspheres described in this patent to molecules or cells has been improved, it is still insufficient, and the preparation process of the magnetic microspheres described in this patent is too complicated, the reaction time is long, and there are many kinds of reagents added.

Method used

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  • Preparation method of one-dimensional magnetic nanowires for biochemical analysis
  • Preparation method of one-dimensional magnetic nanowires for biochemical analysis
  • Preparation method of one-dimensional magnetic nanowires for biochemical analysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for preparing a one-dimensional magnetic nanowire for biochemical analysis, comprising the following steps:

[0027] (1) Preparation of Component A and Component B: Mix 0.5mol / L nickel chloride aqueous solution and ethylene glycol in a ratio of 1:8 by volume to form component A; mix sodium hydroxide powder with ethylene glycol Diols are mixed in a ratio of 1:96 by weight, stirred with a magnetic stirrer, and then the reducing agent hydrazine monohydrate is added until the concentration of hydrazine monohydrate is 0.05mol / L, and the stirring is stopped to obtain component B;

[0028] (2) Preparation of nickel nanowires: Component B is placed under a uniform magnetic field of 0.02T, and component A is slowly added dropwise to component B while stirring to react. The volume ratio of component B to component A is 5: 1. The reaction temperature is 60°C, react for 10 minutes, then use a magnet to collect the nickel nanowires and wash them with distilled water for 3 t...

Embodiment 2

[0032] A method for preparing a one-dimensional magnetic nanowire for biochemical analysis, comprising the following steps:

[0033] (1) Preparation of component A and component B: mix 1mol / L nickel chloride aqueous solution and ethylene glycol in a ratio of 1:9 by volume, which is component A; mix sodium hydroxide powder with ethylene glycol Alcohols are mixed in a ratio of 4:96 by weight, stirred with a magnetic stirrer, and then the reducing agent hydrazine monohydrate (N 2 h 4 ·H 2 O) until the concentration of hydrazine monohydrate is 0.2mol / L, stop stirring to obtain component B;

[0034] (2) Preparation of nickel nanowires: Component B is placed under a uniform magnetic field of 0.05T, and component A is slowly added dropwise to component B while stirring to react. The volume ratio of component B to component A is 9: 1. The reaction temperature is 90°C, react for 10 minutes, then collect the nickel nanowires with a magnet and wash them with distilled water for 8 time...

Embodiment 3

[0040] A method for preparing a one-dimensional magnetic nanowire for biochemical analysis, comprising the following steps:

[0041] (1) Preparation of component A and component B: mix 1mol / L nickel chloride aqueous solution and ethylene glycol in a ratio of 1:9 by volume, which is component A; mix sodium hydroxide powder with ethylene glycol Alcohols are mixed in a ratio of 8:96 by weight, stirred with a magnetic stirrer, and then the reducing agent hydrazine monohydrate (N 2 h 4 ·H 2 O) until the concentration of hydrazine monohydrate is 0.25mol / L, stop stirring to obtain component B;

[0042] (2) Preparation of nickel nanowires: Place component B under a uniform magnetic field of 0.08T, slowly add component A dropwise to component B while stirring to react, and the volume ratio of component B to component A is 12: 1. The reaction temperature is 95°C, react for 10 minutes, and then collect the nickel nanowires with a magnet and wash them with distilled water for 5 times t...

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Abstract

The invention discloses a preparation method of one-dimensional magnetic nanowires for biochemical analysis. The preparation method comprises the following steps of mixing 0.5mol to 1.5mol nickel chloride aqueous solution with ethylene glycol according to a volume ratio of 1:8-10, namely a component A; mixing sodium hydroxide and the ethylene glycol according to a weight ratio of 1-10:96 in proportion and stirring, adding hydrazine hydrate to hydrazine hydrate, wherein the concentration of hydrazine hydrate is 0.05-0.3 mol / L, namely a component B; and then reacting the component A with the component B to generate a nickel nanowire, carrying out coating and amination or carboxylation on the SiO2 to prepare the one-dimensional magnetic nanowire. Compared with magnetic microspheres in the prior art, the specific magnetization intensity of the one-dimensional magnetic nanowires is improved in order, the magnetic capture separation efficiency is high, the specific surface area is obviouslyincreased, the detection sensitivity is obviously enhanced, in addition, a multi-target marker can be provided, and a basic material for developing a multi-marker simultaneous detection method is provided.

Description

technical field [0001] The invention belongs to the field of biochemical materials, in particular to a method for preparing a one-dimensional magnetic nanowire for biochemical analysis. Background technique [0002] In the field of biochemical analysis, such as cell enrichment, protein separation and purification, immunoassay, in vitro detection, etc., magnetic materials are used as carriers to efficiently separate or detect specific components from samples after labeling specific functional molecules on their surface. A specific and efficient method. For example, commercialization began in the 1980s For example, immunomagnetic microspheres can be obtained by combining specific antibodies with biological activity on the surface of magnetic microspheres. Under the action of an external magnetic field, the cells can be quickly and effectively separated or processed by using the combination of antibodies and cells. immunoassay. However, the sensitivity of cell separation or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24B22F1/02B82Y30/00B82Y40/00
Inventor 杨学峰
Owner 珠海启尼亚生物技术有限公司
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