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Bacillus velesi gual203 and its application

A technology of Bacillus and Veles, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of pesticide residue environment, increased dosage of pesticides in the field, pollution, etc.

Active Publication Date: 2022-07-05
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, tobacco black shank and tobacco bacterial wilt often co-occur in Guizhou, and there is no report on the screening of Bacillus that can control these two diseases at the same time
[0004] In addition, capsicum anthracnose is one of the main diseases in capsicum production, which can cause damage throughout the growth period of capsicum, causing great losses
Guizhou Province is one of the main cultivation areas of pepper in my country. The long-term continuous cropping of pepper has caused the continuous expansion of the incidence of anthracnose, seriously affecting the income of farmers
At present, chemical pesticides are mainly used to prevent and control diseases in production. The resistance of pathogenic bacteria is increasing year by year, and the amount of pesticides used in the field is also increasing accordingly, which can easily cause pesticide residues and environmental pollution.

Method used

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  • Bacillus velesi gual203 and its application
  • Bacillus velesi gual203 and its application
  • Bacillus velesi gual203 and its application

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Experimental program
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Embodiment Construction

[0017] The invention is further introduced below in conjunction with the accompanying drawings and specific embodiments:

[0018] 1. Isolation and screening of Bacillus velesi GUAL203

[0019] 1. Materials and methods

[0020] 1.1 Materials

[0021] Soil samples were collected from the rhizosphere soil samples of healthy tobacco plants from Anlong County, Qianxinan Prefecture, Guizhou Province from 2017 to 2018, respectively. Mix to count as 1 soil sample. Use paper bags to collect soil samples, and sterilize soil collection tools in advance to avoid soil microbial contamination.

[0022] 1.2 Culture medium

[0023] LB medium is used for Bacillus isolation and preservation and plate confrontation experiments; PDA medium is mainly used in plate confrontation experiments.

[0024] 1.3 Tobacco disease strains

[0025] The test strains Phytophthora nicotianae and Ralatonia solanacearum were provided by the Department of Plant Pathology, College of Agriculture, Guizhou Univer...

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Abstract

The invention discloses a Bacillus velesi GUAL203, which is preserved in the China Type Culture Collection, and the preservation number is CCTCC NO: M2018870. The invention also discloses the application of Bacillus velesi GUAL203 in simultaneously preventing and treating tobacco black shank and tobacco bacterial wilt. The Bacillus velesi GUAL203 of the present invention has obvious ability to antagonize tobacco black shank and bacterial wilt bacteria at the same time, as well as the ability to antagonize pepper anthracnose.

Description

technical field [0001] The invention relates to Bacillus velesi GUAL203 and its application, and belongs to the technical field of biological control. Background technique [0002] Tobacco blackleg and tobacco bacterial wilt are the most destructive soil-borne diseases in tobacco production in my country, causing huge economic losses every year. Guizhou Province is one of the main producing areas of tobacco production in my country, and the occurrence of tobacco soil-borne diseases is also particularly high. Serious, the annual economic loss caused by the disease is nearly 100 million yuan, which seriously affects the economic income of tobacco farmers. At present, the prevention and control of this disease in production mainly adopts the comprehensive control measures of planting disease-resistant (resistant) varieties and cultivation and control, supplemented by chemical control. . Therefore, at present, most of the chemical control methods are used to control the disease...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00A01P3/00C12R1/07
Inventor 彭丽娟丁海霞余水
Owner GUIZHOU UNIV