Bacillus velesi gual203 and its application
A technology of Bacillus and Veles, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of pesticide residue environment, increased dosage of pesticides in the field, pollution, etc.
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[0017] The invention is further introduced below in conjunction with the accompanying drawings and specific embodiments:
[0018] 1. Isolation and screening of Bacillus velesi GUAL203
[0019] 1. Materials and methods
[0020] 1.1 Materials
[0021] Soil samples were collected from the rhizosphere soil samples of healthy tobacco plants from Anlong County, Qianxinan Prefecture, Guizhou Province from 2017 to 2018, respectively. Mix to count as 1 soil sample. Use paper bags to collect soil samples, and sterilize soil collection tools in advance to avoid soil microbial contamination.
[0022] 1.2 Culture medium
[0023] LB medium is used for Bacillus isolation and preservation and plate confrontation experiments; PDA medium is mainly used in plate confrontation experiments.
[0024] 1.3 Tobacco disease strains
[0025] The test strains Phytophthora nicotianae and Ralatonia solanacearum were provided by the Department of Plant Pathology, College of Agriculture, Guizhou Univer...
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