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Liver cancer targeted ginsenoside-carrying CK chitosan polymer micelle drug delivery system and preparation method thereof

A technology of chitosan polymers and ginsenosides, which is applied in pharmaceutical formulations, antineoplastic drugs, drug combinations, etc., can solve the problems that the targeting of cancer cells needs to be improved, and achieve the effect of improving targeting performance and enhancing effectiveness

Inactive Publication Date: 2019-05-24
HUAIYIN TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, cancer cell targeting of such nanodrug delivery systems remains to be improved

Method used

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  • Liver cancer targeted ginsenoside-carrying CK chitosan polymer micelle drug delivery system and preparation method thereof
  • Liver cancer targeted ginsenoside-carrying CK chitosan polymer micelle drug delivery system and preparation method thereof
  • Liver cancer targeted ginsenoside-carrying CK chitosan polymer micelle drug delivery system and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] According to the following method, the liver cancer-targeted ginsenoside CK-loaded chitosan polymer micelle drug delivery system of Example 1 was prepared:

[0043] (1) Preparation of deoxycholic acid-carboxymethyl chitosan polymer base carrier:

[0044] Weigh 10 mg of deoxycholic acid powder, add it into 5 ml of anhydrous DMSO, stir to dissolve, add 1.5 times the molar amount of EDC and NHS, and stir at room temperature in the dark to obtain deoxycholic acid NHS active ester solution. In addition, 0.5 g of carboxymethyl chitosan was weighed and dissolved in 200 ml of 75% methanol solution, and deoxycholic acid NHS active ester solution was added dropwise, and reacted at room temperature for 36 hours in the dark. The solution obtained by the reaction is dialyzed and freeze-dried to obtain the deoxycholic acid-carboxymethyl chitosan polymer base carrier.

[0045] (2) Preparation of A54 polypeptide modified drug delivery carrier:

[0046] Dissolve 10mg of A54 polypeptide ...

experiment example 2

[0052] According to the following method, the liver cancer-targeted ginsenoside CK-loaded chitosan polymer micelle drug delivery system of Example 1 was prepared:

[0053] (1) Preparation of deoxycholic acid-carboxymethyl chitosan polymer base carrier:

[0054] Referring to the method in Example 1, the deoxycholic acid-carboxymethyl chitosan polymer base carrier was prepared.

[0055] (2) Preparation of A54 polypeptide modified drug delivery carrier:

[0056] Referring to the method of Example 1, a carboxymethyl chitosan polymer drug delivery carrier modified by the A54 polypeptide was obtained.

[0057] (3) Preparation of liver cancer-targeted ginsenoside CK-loaded chitosan polymer micelles drug delivery system:

[0058] Weigh 20 mg of A54 polypeptide-modified carboxymethyl chitosan polymer freeze-dried powder and disperse it in 10 ml of aqueous solution, take 2 mg of ginsenoside CK and dissolve it in 1 ml of methanol, slowly drop into the above solution under rapid stirrin...

experiment example 3

[0061] According to the following method, the liver cancer-targeted ginsenoside CK-loaded chitosan polymer micelle drug delivery system of Example 1 was prepared:

[0062] (1) Preparation of deoxycholic acid-carboxymethyl chitosan polymer base carrier:

[0063] Referring to the method in Example 1, the deoxycholic acid-carboxymethyl chitosan polymer base carrier was prepared.

[0064] (2) Preparation of A54 polypeptide modified drug delivery carrier:

[0065] Referring to the method of Example 1, a carboxymethyl chitosan polymer drug delivery carrier modified by the A54 polypeptide was obtained.

[0066] (3) Preparation of liver cancer-targeted ginsenoside CK-loaded chitosan polymer micelles drug delivery system:

[0067] Weigh 20mg of A54 polypeptide-modified carboxymethyl chitosan polymer freeze-dried powder and disperse it in 10ml of aqueous solution, take 4mg of ginsenoside CK and dissolve it in 1ml of methanol, slowly drop into the above solution under rapid stirring, s...

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Abstract

The invention provides a liver cancer targeted ginsenoside-carrying CK chitosan polymer micelle drug delivery system and a preparation method thereof. The drug delivery system comprises a targeting head group, a basic carrier and anticancer drug, the targeting head group is A54 polypeptide, the basic carrier is amphipathic polymer, the anticancer drug is ginsenside CK, the targeting head group isconnected with a hydrophobic end of the basic carrier through a connection group to form a drug delivery carrier, and the drug delivery carrier entraps ginsenoside CK to form the drug delivery system.The drug delivery system prepared by the method can obviously improve water solubility of ginsenside CK, can actively target liver cancer cells and is of important significance in clinical treatmentof liver cancer.

Description

technical field [0001] The invention relates to the fields of biotechnology and nano-pharmaceuticals, in particular to a liver cancer targeting ginsenoside CK-loaded chitosan polymer micelles drug delivery system and a preparation method thereof. Background technique [0002] Nano drug delivery system is a new type of drug delivery system developed on the basis of nanotechnology, pharmacy and clinical medicine, and is widely used in the treatment of cancer. Among them, the polymer nanomicelle drug delivery system is a nanometer-sized macromolecular ordered aggregate formed by the self-assembly of amphiphilic polymers in aqueous media, and its hydrophobic inner shell can be used to entrap hydrophobic anticancer drugs , improve the water solubility and stability of the drug, prolong the circulation time in the body, and further improve the effect of cancer treatment. [0003] Ginsenoside CK is the main active ingredient of ginsenoside, which has strong anticancer activity and...

Claims

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Application Information

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IPC IPC(8): A61K9/107A61K47/36A61K47/28A61K31/704A61P35/00
Inventor 胡卫成张建梅沈婷张迹
Owner HUAIYIN TEACHERS COLLEGE
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