Slow virus co-transfection adherent 293T cell device for large-scale cell therapy

A cell therapy and lentivirus technology, applied in tissue cell/virus culture devices, biochemical cleaning devices, enzymology/microbiology devices, etc., can solve the problem of inconvenient removal of cultured cells, unfavorable large-scale culture, and unfavorable cell growth. and other problems, to achieve the effect that is conducive to large-scale culture, meets cell growth conditions, and has a scientific and reasonable structure

Pending Publication Date: 2019-05-24
BEIJING ZHONGYUAN HEJU ECONOMIC & TRADE
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current cell adherence culture device has a small culture capacity, and the cultured cells are not easy to take out, which is not conducive to lar

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Slow virus co-transfection adherent 293T cell device for large-scale cell therapy
  • Slow virus co-transfection adherent 293T cell device for large-scale cell therapy
  • Slow virus co-transfection adherent 293T cell device for large-scale cell therapy

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0022] Example: such as Figure 1-5 As shown, the present invention provides a technical solution, a device for co-transfecting adherent 293T cells with lentivirus for large-scale cell therapy, including a culture device body 1, a universal wheel 2, a culture chamber 3, a door 4, and a chute 5. Electric heating plate 6, incubator 7, roller 8, handle 9, liquid inlet pipe 10, vent pipe 11, drain pipe 12, solenoid valve 13, magnetic ball 14, groove 15, culture plate 16, adherent layer 17 , Cover 18, electromagnet 19, first control box 20, control panel 21, vent 22, fan 23, water pump 24, air pump 25, second control box 26, porous partition 27, temperature sensor 28, controller REX -C90029, timer 30 and controller BF-10T31, the bottom of the culture device body 1 is provided with a universal wheel 2, and the lower side of the culture device body 1 is provided with a culture chamber 3, and a door 4 is installed on the side of the culture chamber 3, In addition, chutes 5 are provide...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a slow virus co-transfection adherent 293T cell device for large-scale cell therapy. The device comprises a culture device body, electric heating plates, an incubator, magneticballs, a culture plate, an adherent layer, electromagnets, a first control box and a second control box. A culture bin is arranged on one side of the lower portion of the culture device body, the electric heating plates are arranged on two inner sides of the culture bin, the magnetic balls are arranged at the inner bottom of the incubator, the culture plate is arranged in a groove, the electromagnets are uniformly arranged at the bottom of a cover plate, a fan is mounted on one inner side of the first control box, a controller REX-C900 is arranged on one side of a temperature sensor, and a controller BF-10T is arranged on one side of a timer. The incubator is used for culture, the adherence area of cells is increased by the culture plate, the culture plate is easily taken out, cell growthconditions are met, large-scale culture is facilitated, the magnetic balls are adsorbed by the electromagnets and accordingly move up and down in the incubator, culture solution is stirred, and the cells cannot be injured.

Description

technical field [0001] The invention relates to the technical field of cell adherence culture, in particular to a device for co-transfecting adherent 293T cells with lentivirus for large-scale cell therapy. Background technique [0002] 293T cell is a cell line derived from 293 cells through genetic technology. It is transfected with adenovirus E1A gene and can express SV40 large T antigen. It contains SV40 replication origin and promoter region. It is a very commonly used expression research tool The cells of the source gene are of great significance for the use of gene therapy for diseases, and 293T cells are usually propagated by cell adherent culture. [0003] However, the current cell adherence culture device has a small amount of culture, and the cultured cells are not easy to take out, which is not conducive to large-scale culture. When culturing the cells, stirring is required, which is easy to cause damage to the cultured cells, which is not conducive to the growth ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12M3/00C12M1/38C12M1/02C12M1/00
Inventor 朱希灿
Owner BEIJING ZHONGYUAN HEJU ECONOMIC & TRADE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap