A low-modification high-viscosity hyaluronic acid gel and its preparation method and application
A hyaluronic acid, high-viscosity technology, applied in surgery, medical science, etc., can solve problems such as low complex modulus value, high cross-linking agent usage, low initial reaction concentration of hyaluronic acid polymer, etc.
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Embodiment 1
[0040] Embodiment 1 High molecular weight sodium hyaluronate prepares sodium hyaluronate gel under low initial reaction concentration conditions
[0041] 0.6g of sodium hyaluronate with molecular weight of 2.52MDa and 0.6g of molecular weight of 0.86MDa were dissolved in 1% sodium hydroxide solution respectively to obtain 6mg / mL sodium hyaluronate solution A and 30mg / mL sodium hyaluronate Solution B. Add 1,4-butanediol and glycidyl ether with a volume weight ratio of 0.06-0.18 between the cross-linking agent and sodium hyaluronate A to the sodium hyaluronate solution A and fully dissolve it. Put it in a water bath at 42°C for 3 hours before adding Hyaluronic acid solution B and mix evenly, let it stand at 7-10°C for 40 hours. Wash the gel several times with a large amount of phosphate buffer, and swell until it no longer absorbs water, and the obtained gel is sterilized by high-temperature steam at F0=12. The final sodium hyaluronate content of the gel was detected to be 5.4...
Embodiment 2
[0042] Example 2 Preparation of Sodium Hyaluronate Gel by High Molecular Weight Sodium Hyaluronate under Low Initial Reaction Concentration Conditions
[0043] Dissolve 1.0 g of sodium hyaluronate with a molecular weight of 2.52 MDa and 0.84 g of a molecular weight of 0.86 MDa in 1% sodium hydroxide solution to obtain 10 mg / mL sodium hyaluronate solution A and 42 mg / mL sodium hyaluronate Solution B. Add 1,4-butanediol and glycidyl ether with a volume weight ratio of 0.06-0.18 between the cross-linking agent and sodium hyaluronate A to the sodium hyaluronate solution A and fully dissolve it. Put it in a water bath at 42°C for 3 hours before adding Hyaluronic acid solution B and mix evenly, let it stand at 7-10°C for 40 hours. Wash the gel several times with a large amount of phosphate buffer, and swell until it no longer absorbs water, and the obtained gel is sterilized by high-temperature steam at F0=12. The final sodium hyaluronate content of the gel was detected to be 6.9 ...
Embodiment 3
[0044] Example 3 High Molecular Weight Sodium Hyaluronate Prepares Sodium Hyaluronate Gel Under Low Initial Reaction Concentration Conditions
[0045] Dissolve 1.5 g of sodium hyaluronate with a molecular weight of 2.52 MDa and 0.84 g of a molecular weight of 0.86 MDa in 1% sodium hydroxide solution to obtain 15 mg / mL sodium hyaluronate solution A and 42 mg / mL sodium hyaluronate Solution B. Add 1,4-butanediol and glycidyl ether with a volume weight ratio of 0.06-0.18 between the cross-linking agent and sodium hyaluronate A to the sodium hyaluronate solution A and fully dissolve it. Put it in a water bath at 42°C for 3 hours before adding Hyaluronic acid solution B and mix evenly, let it stand at 7-10°C for 40 hours. Wash the gel several times with a large amount of phosphate buffer, and swell until it no longer absorbs water, and the obtained gel is sterilized by high-temperature steam at F0=12. The final sodium hyaluronate content of the gel was detected to be 7.3 mg / mL, an...
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