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Inducing activation kit for DC-CTL cells and application method thereof

A technology of DC-CTL and application method, which is applied in the field of kits or kits, can solve the problems of inconsistent reagent standards, long incubation time, and high risk of bacterial infection, and achieve the effects of uniform quality, shortened time, and convenient use

Inactive Publication Date: 2019-05-31
苏州明基医院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In recent years, the research and development of DC-CTL has been deepened, and a relatively mature DC-CTL induction and culture method has been formed. However, many reagents used in the cell culture process have inconsistent standards, and the workload in the culture process is heavy. Long time and high risk of bacterial infection make it difficult for many researchers to cultivate DC-CTL cells with uniform quality in a short period of time

Method used

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  • Inducing activation kit for DC-CTL cells and application method thereof
  • Inducing activation kit for DC-CTL cells and application method thereof

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Embodiment Construction

[0032] In order to have a further understanding of the purpose, structure, features, and functions of the present invention, the following detailed descriptions are provided in conjunction with the embodiments.

[0033] Certain terms are used in the description and claims to refer to particular elements. Those of ordinary skill in the art will appreciate that manufacturers may refer to the same element by different terms. The specification and claims do not use the difference in name as a way to distinguish components, but use the difference in function of components as a criterion for distinguishing. "Include" mentioned throughout the specification and claims is an open term, so it should be interpreted as "including but not limited to".

[0034] Such as figure 1 with figure 2 As shown, the DC-CTL cell induction and activation kit of the present invention is composed of a box body 1, a box cover 2, a sponge holder 3, and a reagent bottle 4. The box body 1 and the box cove...

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Abstract

The invention discloses an inducing activation kit for DC-CTL cells and an application method thereof. The kit comprises a lid and a body, wherein the body is internally provided with a sponge holderprovided with six fixing holes. The six fixing holes are respectively provided with a reagent bottle containing a CTL cytokine activator IL2, a reagent bottle containing a modulating CTL cytokine activator IFN-gamma, a reagent bottle containing a mixed solution of modulating DC cytokine activators IL-4 and GM-CSF, a reagent bottle containing a modulating DC cytokine activator TNF-alpha, a reagentbottle containing a lymphocyte separation solution and a reagent bottle containing a serum-free culture solution. Therefore, the inducing activation kit of the DC-CTL cells is simple, practical and convenient. The reagent bottle is of a split structure, convenient to clean and disinfect, capable of reducing the risk of contamination in the culture process and capable of culturing DC-CTL cells uniform in quality within short time.

Description

technical field [0001] The invention relates to the technical field of kits or kits, in particular to a kit for inducing and activating DC-CTL cells and an application method thereof. Background technique [0002] Tumor biotherapy is a hot spot in the field of tumor research today, among which tumor adoptive immunotherapy occupies an important position in tumor biotherapy. It has a good effect on promoting the reconstruction of the patient's immune system, eliminating minimal residual disease, and purifying the bone marrow. A key issue in adoptive immunotherapy of tumors is to find suitable tumor-killing cells. After a long period of exploration, it has been recognized that the basis of tumor immunity is the existence of tumor-specific antigens and the effective response of the immune system to tumor cells, and that cellular immunity plays a key role in tumor immunotherapy, especially with antigen presentation The ability of dendritic cells (DC, dendritic cell) and CTL cel...

Claims

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Application Information

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IPC IPC(8): C12N5/0783C12N5/0784
Inventor 杨光
Owner 苏州明基医院有限公司
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