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Method of directly and simultaneously detecting nitrate and nitrite in exhaled breath condensate

A technology of exhaled breath condensate and nitrite, applied in the field of nitrate and nitrite, can solve the problems of matrix color interference, cumbersome operation, etc., and achieve the effect of less sample volume, simple and fast operation, and high sensitivity

Inactive Publication Date: 2019-06-04
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] The above two methods are cumbersome to operate and can only detect nitrite in the sample. If you need to detect nitrate, you need to first reduce the nitrate in the sample to nitrite, and then measure the nitrate and nitrous acid in the sample. The total amount of salt, then measure the content of nitrite, subtract the two to get the content of nitrate
In addition, when using photometry, it is also interfered by the color of the matrix

Method used

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  • Method of directly and simultaneously detecting nitrate and nitrite in exhaled breath condensate
  • Method of directly and simultaneously detecting nitrate and nitrite in exhaled breath condensate
  • Method of directly and simultaneously detecting nitrate and nitrite in exhaled breath condensate

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Embodiment

[0024] Collect 1mL of exhaled breath condensate, add 20 L of 3% phosphoric acid dropwise, shake and mix evenly. Take 5L of sample and drop it in the groove of the sampling carrier, insert the sampling carrier into the thermal desorption cavity, and the desorption temperature is 180°C. Obtain the spectrogram corresponding to the peak height of nitrate and nitrite changing with the desorption time as figure 2 shown. The signal intensity varies with migration time and thermal desorption time as image 3 shown.

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Abstract

The invention provides a method of directly and simultaneously detecting nitrate and nitrite in an exhaled breath condensate. Based on thermal desorption ion mobility spectrometry, phosphoric acid isadded to the collected exhaled breath condensate for acidification and is then dropped on a thermal desorption carrier for thermal desorption detection, and as nitric acid, nitrous acid and a substrate have different desorption time and different mobility, simultaneous quick detection on nitrate and nitrite in a complex biological sample is realized.

Description

technical field [0001] The invention relates to a method for simultaneously detecting nitrate and nitrite, in particular to a method for simultaneously detecting nitrate and nitrite in exhaled breath condensate by thermal desorption ion mobility spectrometry. Background technique [0002] Nitrate and nitrite in exhaled breath condensate (EBC) can be used as biomarkers to aid in disease diagnosis. [0003] For example, the increase of nitrite content in EBC is closely related to various diseases such as asthma, cystic fibrosis, chronic obstructive pulmonary disease, and acute gastric loss. While elevated nitrate levels are closely associated with asthma and community-acquired pneumonia, nitrate levels are not elevated in chronic obstructive pulmonary disease. Therefore, for different diseases, the simultaneous detection of nitrate and nitrite can help doctors diagnose diseases more accurately. [0004] The commonly used detection methods for nitrite and nitrate in EBC repor...

Claims

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Application Information

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IPC IPC(8): G01N27/62
Inventor 李海洋陈红陈创厉梅王卫国
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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