31 results about "Exhaled breath condensate" patented technology

The present invention provides systems and methods for analyzing glucose present in exhaled breath condensate (EBC). In certain embodiments of the invention, electrochemical- or coulometric-based sensing technologies are used to analyze EBC for the presence and/or concentration of glucose.

A method and system that provides for minute-to-minute EBC pH monitoring (or for other EBC characteristic monitoring) of a subject that greatly assists in determining the time-course of airway pH changes (or other characteristic changes) in evolving disease processes, and may assist in determining the predictive ability of these tests, as well as determining response to therapy.

The present invention relates to noninvasive methods for obtaining exhaled breath condensate (EBC) samples from the airways and lungs of a subject for use in diagnosing various conditions and diseases associated with biomarkers present in EBC, including diagnosis of lung cancer. The methods of the subject invention include generating and/or applying an oscillating airflow to a subject during inhalation and/or exhalation to induce increased concentration of biomarkers in EBC; collecting EBC from exhaled breath; and analyzing the collected EBC for biomarkers associated with disorders and/or diseases.

The present invention includes an apparatus and a method for testing exhaled breath condensate for at least one biometric marker, including a substrate for collecting exhaled breath condensate, the substrate capable of being brought to a temperature below a dew point of vapor in exhaled breath, a collector for retaining the substrate and for receiving a biometric marker reagent. The invention may further include receiving exhaled breath in a collector, the collector comprising a substrate, the substrate being at a temperature below a dew point of vapor in exhaled breath, collecting the exhaled breath condensate on the substrate, and bringing a biometric marker reagent in contact with the substrate.

The invention relates to a EBC detection as a detection technique completely without wound, with important use value for airway inflammation. A fourway gaspath solves the automatic separation of entrance of expired air, exit of expired air, inspired air and spit residue. The upbending design and 'claw type' of the exit pipeline of the expired air can solve the effect of spit residue from the expired air on refrigeration area. A singlechip system detects the expired air amount and the condensing temperature is set to quantify the collection of the condensate of expired air, so as to quantitatively evaluate change and degree of states such as oxidation stress, inflammation and the like in airway of a variety of respiratory disease.

A system and method for detecting a biomarker in exhaled breath condensate nanodroplets comprises noninvasively collecting exhaled breath condensate nanodroplets of a subject, and analyzing said nanodroplets utilizing immuno-quantitative polymerase chain reaction to detect one or more target biomarkers.

The invention discloses an exhaled breath condensate collector. The collector comprises a refrigeration system and a condensate collecting system; the refrigeration system comprises a temperature control box and a condensation tube sleeve, and the outer wall of the condensation tube sleeve is provided with an evaporator; the condensate collecting system comprises a breath inlet nozzle, an inhalation and exhalation four-way control valve, a condensation tube and a collecting cup, the inhalation and exhalation four-way control valve comprises a breath inlet nozzle connector, an inhalation inlet,an exhalation outlet and a condensation tube connector, and the collecting cup sleeves the bottom of an outer tube of a condensation tube; during use, the condensation tube sleeve sleeves the outer wall of the condensation tube and the collecting cup. The exhaled breath condensate collector is convenient to mount and dismount, the refrigeration system and the condensate collecting system are structurally independent, a patient is subjected to exhaled breath condensate detection without sitting up, and the collector is friendly to users.

A device for collecting exhaled breath condensate from a subject. The device comprises a plunger assembly and a stopper. The stopper is connected to the plunger disk of the plunger assembly by a plurality of support pins and is configured for sealing engagement. The device is utilized to collect exhaled breath condensate from both spontaneously breathing and mechanically ventilated subjects and the devices utilized to determine whether lipopolysaccharide is present in the collected exhaled breath condensate.

The invention discloses application of ezrin in preparing an asthma diagnostic reagent. From exhaled breath condensate (EBC)and serum (serum) of a healthy person and an asthma patient, it is found that the ezrin level of the healthy person can be remarkably differentiated from that of the asthama patient. The EBC and the ezrin level in the serum of the asthma patient are remarkably declined, and the EBC and the ezrin level in the serum have positive correlations with a lung function and have negative correlations with IL-13 and a periosteum peotein level in the serum, therefore, by monitoring the ezrin level in the human body serum (serum) or the exhaled breath condensate (EBC), diagnosis of the asthma can be conducted, treatment of the asthma is assisted, and prognosis of the asthma is evaluated. According to the application of the ezrin in preparing the asthma diagnostic reagent, guiding functions can be achieved in aspects of prediction, diagnosis and evaluation of a bronchial asthma disease through a simpler and more convenient method, and air passage barrier function changing conditions of the asthma patient can be more sensitively reflected. Based on the function of a biomarker of the ezrin, a more reliable and more sensitive kit can be hopefully prepared, asthma controlling conditions can be directly reflected, and treatment and prognosis evaluating of the asthma are guided.

The invention provides a method of directly and simultaneously detecting nitrate and nitrite in an exhaled breath condensate. Based on thermal desorption ion mobility spectrometry, phosphoric acid isadded to the collected exhaled breath condensate for acidification and is then dropped on a thermal desorption carrier for thermal desorption detection, and as nitric acid, nitrous acid and a substrate have different desorption time and different mobility, simultaneous quick detection on nitrate and nitrite in a complex biological sample is realized.

An electrochemical method and an electrochemical sensor for breath analysis of single or multiple analytes using a porous, preferably flexible and disposable supporting material is provided, a salt is incorporated, and which can be wetted in contact with the exhaled breath condensate. The electrochemical method acting simultaneously as sampling method, as an electrolyte and as a support for the electrode structures. In some embodiments the salt may be hygroscopic, such that the porous substrate stays wet. To ensure that the obtained signal originates from the analyte, the electrochemical sensor preferably exhibits a differential electrode design, including a sensing (analyte-sensitive) and a blank (analyte-insensitive) electrode in order to isolate and remove the background signals.

An exhaled breath condensate collection device (10) is described. The device includes a condensation chamber (12) having an inlet and an outlet and arranged such that breath exhaled from the lungs of a user flows through the condensation chamber from the inlet to the outlet; at least one cooling element (16); and a frame (18) supporting the condensation chamber and the at least one cooling element. The frame (18) is movable between a first configuration in which the at least one cooling element is operable to cool the condensation chamber so as to promote the formation of breath condensate in the condensation chamber, and a second configuration in which the cooling element is displaced from the condensation chamber so as to encourage condensate to flow into a collection region in fluid communication with the condensation chamber. To be accompanied by FIG. 5.

The invention discloses a portable sampler for exhaled breath condensate and a sampling method. A semiconductor chilling plate is utilized to provide a low-temperature condensing environment, and a sterile disposable suction tube and a 50ml centrifugal tube are utilized to collect the exhaled breath condensate of a human body. The sampler is simple in structure and portable in volume; the sampling method is simple in operation; except for an external power supply, the portable sampler does not need other consumable items and auxiliary equipment which are difficult to carry, such as a refrigerant; the exhaled breath condensate of the human body, which is enough for analyzing the disease infection condition, can be noninvasively and quickly collected; meanwhile, the discomfort and cross infection of a testee and the cross contamination of a sample can be avoided to the greatest extent; and the portable sampler is suitable for large-scale sampling detection diagnosis and epidemiological investigation of people infected by respiratory pathogens.

A method is provided for determining pulmonary embolism and/or increased risk thereof in a human being, comprising collecting a sample of exhalation air from said human being and determining the presence or absence in said exhaled air of one or more biomarkers associated with pulmonary embolism. Additionally, a kit that comprises the means for detecting at least one biomarker associated with pulmonary embolism or risk thereof is provided.

The present invention relates to roadside analyzer for determination of illegal drugs abuse, including, but not limiting to detection of explosives, toxic industrial chemicals and other banned or regulated compounds, biomarkers and phytochemicals in a sample in situ in at least one human body fluid sample, specifically in oral fluid (saliva), but not limiting to other clinical samples of interest (urine, blood, exhaled breath, exhaled breath condensate, etc.) It consists of automatic processor for preparing samples suitable for analysis. Analysis part of the instrument implements three technologies, namely solid phase extraction prior to analysis, capillary electrophoresis for separation of analytes from the sample matrix and impedance (contactless conductivity) or fluorescence or both impedance (contactless conductivity) and fluorescence for detection of analytes of interest.

An exhaled breath condensate (EBC) collection device comprising two adjacent parts foldable together and apart from each other, a condensation zone to condense exhaled breath from a subject, the condensation zone being a third part provided between the two adjacent parts and connectable to cooling means arranged in each of the two parts being in cooling relationship with the condensation zone. There is also provided a method for detecting antigens or antibodies to the antigens, the method comprising collecting EBC from a subject using the EBC collection device, performing immunochromatographic testing (ICT) of the collected exhaled breath condensate, and interpreting the immunochromatographic testing results as follows: if the immunochromatographic testing results are positive, the antigens or antibodies to the antigens are detected, or if the immunochromatographic testing results are negative, the antigens or antibodies to the antigens are not detected.