Application of ezrin in preparing asthma diagnostic reagent

A technology of 1. ezrin and diagnostic reagents, applied in the direction of biological testing, material inspection products, etc., can solve problems such as the lack of plasma markers, and achieve the effects of simple diagnosis and evaluation, changes in sensitive airway barrier function, and simple methods

Active Publication Date: 2017-04-26
JIANGSU PROVINCE HOSPITAL
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At present, there are many clinical studies on the single or combined use of plasma biomarkers as a means of disease di

Method used

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  • Application of ezrin in preparing asthma diagnostic reagent
  • Application of ezrin in preparing asthma diagnostic reagent
  • Application of ezrin in preparing asthma diagnostic reagent

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Embodiment Construction

[0014] The present invention will be further described below in conjunction with specific embodiments and drawings.

[0015] Serum collection of healthy people and asthmatic patients:

[0016] Collected 61 patients with asthma confirmed by lung function test, including 25 males and 36 females. According to the 2015 GINA ACT scale (asthma control test form), asthma patients were divided into three types: fully controlled, partially controlled, and uncontrolled. Large category; 23 cases in the control group, all healthy adults. Collect venous blood from asthma patients and healthy people. Immediately after collection, centrifuge the blood samples at 3000 rpm for 10 minutes to separate the serum. Place the separated serum samples in labeled test tubes and store them in an ultra-low temperature refrigerator below -80°C. Collect the exhaled air condensate (EBC) of asthma patients and healthy people for 15 minutes, measure the amount, and store it in a -80°C freezer.

[0017] Detection m...

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Abstract

The invention discloses application of ezrin in preparing an asthma diagnostic reagent. From exhaled breath condensate (EBC)and serum (serum) of a healthy person and an asthma patient, it is found that the ezrin level of the healthy person can be remarkably differentiated from that of the asthama patient. The EBC and the ezrin level in the serum of the asthma patient are remarkably declined, and the EBC and the ezrin level in the serum have positive correlations with a lung function and have negative correlations with IL-13 and a periosteum peotein level in the serum, therefore, by monitoring the ezrin level in the human body serum (serum) or the exhaled breath condensate (EBC), diagnosis of the asthma can be conducted, treatment of the asthma is assisted, and prognosis of the asthma is evaluated. According to the application of the ezrin in preparing the asthma diagnostic reagent, guiding functions can be achieved in aspects of prediction, diagnosis and evaluation of a bronchial asthma disease through a simpler and more convenient method, and air passage barrier function changing conditions of the asthma patient can be more sensitively reflected. Based on the function of a biomarker of the ezrin, a more reliable and more sensitive kit can be hopefully prepared, asthma controlling conditions can be directly reflected, and treatment and prognosis evaluating of the asthma are guided.

Description

Technical field [0001] The invention belongs to the field of detection reagents, and particularly relates to the application of a protein in preparing an asthma diagnostic reagent. Background technique [0002] Bronchial asthma is abbreviated as asthma, which refers to the chronic inflammation of the airway that involves a variety of cells (such as eosinophils, mast cells, T lymphocytes, neutrophils, airway epithelial cells, etc.) and cellular components. Sexual disease. Asthma is one of the most common chronic diseases in the world. It can widely affect all ages. The prevalence of asthma in various countries ranges from 1% to 18%. In my country, it is about 0.5% to 5%, and it is showing an upward trend year by year. It has become a heavy burden for global health management. The mortality rate of asthma is 1.6 to 36.7 per 100,000, which is mostly caused by long-term poor control of asthma and delayed treatment during acute attacks, and most of these are preventable and controlla...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 姚欣贾嫚蒋昕钰姜洁欧英炜张秀伟单霞黄茂
Owner JIANGSU PROVINCE HOSPITAL
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