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A compound improver for saline-alkali land and its application method

A technology of saline-alkali land and soil improver, which is applied in the field of soil remediation, can solve the problems of little application value and promotion significance, reduce the salt content of saline-alkali land, and cannot convert saline-alkali land, so as to increase the level of plant growth hormone, inhibit growth, and strengthen improvement. effect of effect

Active Publication Date: 2021-09-28
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the soil salinity is too high, the growth of salt-tolerant plants and halophytes will also be inhibited; and this method can only reduce the salt content of saline-alkali land to a certain extent, and cannot completely convert saline-alkali land into arable land, so , the practical application value and promotion significance are not great

Method used

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  • A compound improver for saline-alkali land and its application method
  • A compound improver for saline-alkali land and its application method
  • A compound improver for saline-alkali land and its application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Screening and Identification of the required microorganism

[0047] 1. The medium and reagents according to the present embodiment are as follows:

[0048] (1) Beef paste protein liquid medium: protein 胨 10g, beef paste 3g, sodium chloride 5g, distilled water 1000mL, adjusted pH = 7.0, 121 ° C for 20 min. A 18 g of agar was added to beef paste protein liquid medium, which was beef paste protein.

[0049] (2) ASHBY no nitrogen liquid medium: mannitol 10g, Caco 3 5G, KH 2 PO 3 0.2g, MGSO 4 · 7h 2 O0.2G, NaCl 0.2g, CASO 4 · 2h 2 O 0.1 g, pH = 7.4, deionized water 1000ml.

[0050] (3) Polyophosphorus liquid medium: glucose 10g, calcium phosphate 5g, ammonium sulfate, 0.2 g of potassium chloride, 0.25 g of vitrocephal, 1000 ml of distilled water, and 6.8 to 7.0, 115 ° C. Sterile 30 min.

[0051] (4) DF liquid medium: MNSO 4 · 7h 2 O 0.2g, kh 2 PO 4 4.0g, NA 2 HPO 4 6.0g, 2.0g of citric acid, 2.0 g of glucose, 2.0g of sodium gluconate, (NH 4 ) 2 SO 4 2.0g, component 0....

Embodiment 2

[0086] Example 2: Sorted Sorted Selling Selling Base Explanation

[0087] 1. Preparation of seed fluid: The strain YZX4 and YX7 obtained by the examples were inoculated in a beef paste protein-solid medium, respectively: 30 ° C constant temperature incubator was cultured for 8 h. The activated functional strain YZX4 and YX7 to beef paste in a single cycle were collected, and the seed liquid was cultured to the strain of 28 ° C, 180 r / min (determined by the growth curve and the OD value) to obtain a seed liquid.

[0088] 2. Salt resistance test: Take a rings of YZX4 and YX7 bacteria (seed liquid), which are inoculated into 0, 20, 50, 70, 100, 120, 150, 200 g / l NaCl, beef paste protein liquid, respectively. In the medium (pH = 7.2), it was used as a blank control, 30 ° C, 180R / min shaker culture 2D in a white gas medium. Determination of the absorbance value at 600 nm (OD) 600 ).

[0089] Alkali-resistant ability test: Take a secondary endococcus body inoculated into a 20 g / ...

Embodiment 3

[0091] Example 3: Screening of remodeling in modified agent

[0092] 1. Formulated 30 g / l NaCl solution, measured its pH and PNA values, and the measurement is a pH of 5.77, and the PNA value is 0.79. 50 ml of the above solution was taken, and the pH of each bottle solution and the PNA value were measured after 30 ° C, 160 r / min, respectively, at a constant temperature shaker of 30 ° C, 160 r / min, respectively. The desal dehydrate selected from this experiment is D001 resin, AB500-type resin, D113-type resin, and zeolite powder, and three repetitions per desolder.

[0093] 2. The measurement results are shown in Table 3.

[0094] Table 3 Different dehydrate measurement results show

[0095] Type of desalination agent The pH after treatment Processing PNA D001 1.34±0.02 2.02±0.07 D113 2.28±0.03 1.31±0.03 AB500 1.87±0.01 1.50±0.02 Zeolite powder 6.06±0.03 0.93±0.01

[0096] The results showed that the pH of the NaCl solution after D001 wa...

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PUM

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Abstract

The invention belongs to the field of soil restoration, and in particular relates to a compound improver for saline-alkali land and an application method thereof. Specifically, a new Bacillus subtilis is provided, and a soil conditioner is prepared by using it. The specific components of the soil conditioner include: 5-10 parts of microbial agent, 70-80 parts of organic fertilizer, and 10-20 parts of biochar , 5 to 10 parts of desalting agent. And based on the soil improver, a series of methods for improving saline-alkali land are provided. Using the method provided by the invention, there is no secondary pollution, the environment is friendly, and the severe saline-alkali land can be completely improved into land that can be cultivated normally.

Description

Technical field [0001] The present invention belongs to the field of soil restoration, and more particularly to a saline-base composite modified agent and a method of administration thereof. Background technique [0002] Salt accumulation in the soil is a series of natural and human factors that act on different spatial scale to superimpose. Soil salinity has severely restrictive agricultural development, improved significant resource resources, and has extremely important significance for agricultural production development, land governance, and ecological environment protection. [0003] At present, the improvement method of salt bases mainly includes physical law, chemical method, and biological law. The physical method mainly is mainly destroyed, combined with turns, collapse, siltave and other measures to achieve improved salt alkaline earth; chemical method is to reduce organic fertilizer by adding some acid chemicals and organic polymer materials, etc. Soil Saline Alkaliza...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K17/40C09K109/00C09K101/00C12R1/125C12R1/38
Inventor 李东孙晓莹陈意超刘晓风
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S