External application ointment for and application thereof in repairing diabetic foot wounds

A technology for external application of ointment and ointment, applied in the field of ointment, can solve the problems of difficult healing of diabetic foot wounds, achieve the effects of improving tissue repair potential, reducing inflammatory reactions, and inhibiting fibrosis

Inactive Publication Date: 2019-06-11
北京隆祺生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the existing technical problem that diabetic foot wounds are difficult to heal, the present invention provides an externally applied ointment and its application in repairing diabetic foot wounds

Method used

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  • External application ointment for and application thereof in repairing diabetic foot wounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Preparation of exosomes from human umbilical cord mesenchymal stem cells

[0023] Aseptically take the umbilical cord and cut it into small pieces of about 3 cm, draw out and discard the blood vessel, take the perivascular material and cut it into pieces, first use 1‰ type IV collagenase to digest for 2 hours, then centrifuge at 2000r / min for 10 minutes, remove the lower layer of sediment and then use 0.25% pancreatic Continue to digest protease-PBS at 37°C for 25 minutes, then centrifuge at 2000r / min for 10min, take the cell pellet and digest it with 0.25% trypsin solution, then centrifuge at 800r / min for 10min, take the cell pellet and wash it at 1×10 6 The cells / ml density is suspended in the culture medium, and the culture medium is a commercially available serum-free medium for mesenchymal stem cells. Set at 37°C, 100% humidity, 5% CO 2 Cultivate in a dark environment, change the medium every 3 days, after 2 days, fibroblasts can be seen growing at the bottom o...

Embodiment 2

[0028] 1. Preparation of exosomes from human umbilical cord mesenchymal stem cells

[0029] Human umbilical cord mesenchymal stem cell exosomes were prepared by the same method as in Example 1.

[0030] 2. Preparation of topical ointment

[0031] a. 40 parts by weight of the ointment composition are placed in a blender and stirred, wherein the proportions of the ointment composition are respectively 25 parts of aloe juice, 6 parts of catechin powder, and 7 parts of calendula powder by weight , 3 parts of peppermint powder, 5 parts of collagen, 3 parts of vitamin B, 20 parts of ointment, 12 parts of moisturizing agent, 6 parts of emollient, 65 parts of water; the moisturizing agent is a mixture of glycerin and propylene glycol, and the weight of glycerin and propylene glycol The ratio is 5:1, the emollient is a mixture of oleyl alcohol and cholesterol, and the weight ratio of oleyl alcohol to cholesterol is 3:5; the ointment is glycerin. The stirring time of the ointment comp...

Embodiment 3

[0034] 1. Preparation of exosomes from human umbilical cord mesenchymal stem cells

[0035] Human umbilical cord mesenchymal stem cell exosomes were prepared by the same method as in Example 1.

[0036] 2. Preparation of topical ointment

[0037] a. 30 parts by weight of the ointment composition are placed in a blender and stirred, wherein the proportions of the ointment composition are respectively 20 parts of aloe juice, 4 parts of catechin powder, and 6 parts of calendula powder by weight , 2 parts of mint powder, 4 parts of collagen, 2 parts of vitamin B, 15 parts of ointment, 11 parts of moisturizing agent, 5 parts of emollient, 70 parts of water; the moisturizing agent is a mixture of glycerin, propylene glycol and sorbitol, and glycerin, The weight ratio of propylene glycol and sorbitol is 5:1:2; the emollient is a mixture of oleyl alcohol, cholesterol, and hydrogenated palm oil, and the weight ratio of oleyl alcohol, cholesterol, and hydrogenated palm oil is 3:5:8; oi...

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Abstract

The invention relates to the technical field of ointments, in particular to an external application ointment and application thereof in repairing diabetic foot wounds. The external application ointment comprises a mesenchymal stem cell exosome and an ointment composition, wherein the ointment composition comprises aloe juice, catechu, hedyotidous hedyotis stem and leaf and mint. The mesenchymal stem cell exosome contained in the external application ointment for repairing diabetic foot wounds has important biological functions of inhibiting cell apoptosis, relieving inflammatory reaction, promoting angiogenesis, inhibiting fibrosis, improving tissue repair potential and the like, and can be directly used for repairing wounds on the surface of skin. In addition, traditional Chinese medicinecomponents such as aloe juice, catechu, hedyotidous hedyotis stem and leaf and mint contained in the external application ointment have the effects of sterilization, inflammation resistance, detumescence, pain relieving and the like, the pain feeling of a patient can be effectively reduced, and through clinical tests, foot wounds can be effectively repaired, and the treatment period is short.

Description

technical field [0001] The invention relates to the technical field of ointment, in particular to an externally applied ointment and its application in repairing diabetic foot wounds. Background technique [0002] Diabetes is a group of metabolic diseases characterized by hyperglycemia. Hyperglycemia is caused by defective insulin secretion or impaired biological action, or both. The long-term high blood sugar in diabetes leads to chronic damage and dysfunction of various tissues, especially the eyes, kidneys, heart, blood vessels, and nerves. [0003] Diabetic foot is one of the serious complications of diabetes. Its pathogenesis is that due to the long-term impact of high blood sugar in diabetic patients, vascular lesions in the lower extremities cause vascular occlusion and blood supply disorders, resulting in insufficient nutrients for the foot tissues, resulting in edema, Blackening, ulceration and necrosis. In addition, insufficient blood supply to the lower limbs c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/886A61K36/896A61K9/06A61P3/10A61P17/02A61P29/00A61P31/02A61K35/51
Inventor 吴国栋李大军
Owner 北京隆祺生物科技有限公司
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