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Application of hsa-miR-15b-5p as molecular marker for acute high altitude reaction susceptible persons and kit

A technology of hsa-mir-15b-5p and 1.hsa-mir-15b-5p, which is applied in molecular biotechnology and its application fields, and achieves the effects of good screening efficiency, high reliability and large quantity

Active Publication Date: 2019-06-11
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on the correlation between circulating microRNAs and AMS susceptibility.

Method used

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  • Application of hsa-miR-15b-5p as molecular marker for acute high altitude reaction susceptible persons and kit
  • Application of hsa-miR-15b-5p as molecular marker for acute high altitude reaction susceptible persons and kit
  • Application of hsa-miR-15b-5p as molecular marker for acute high altitude reaction susceptible persons and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Correlation between the expression of microRNA in plasma samples and the risk of AMS

[0043] 1. Material and Specimen Collection Description

[0044] Plasma samples of AMS-susceptible individuals were collected from 209 AMS patients who entered the plateau rapidly before entering the plateau. Plasma samples of normal people were collected from 203 normal healthy people who entered the plateau rapidly before entering the plateau. The diagnosis of AMS was confirmed by the International Common Diagnostic Criteria-Lake Lewis Score. All the population did not take any preventive medicine before blood sampling. A total of 2ml of blood was collected in EDTA-Na anticoagulant tubes for each sample. The clinical case characteristics of the patients are shown in Table 1. This study has been approved by the Medical Ethics Committee of the Army Military Medical University (Third Military Medical University), and all specimens were collected with the informed consent o...

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Abstract

The invention relates to a microRNA marker for screening acute high altitude reaction susceptible persons, and the microRNA is an application of hsa-miR-15b-5p as a molecular marker reaction for the acute high altitude reaction susceptible persons, and can be applied to preparation of a reagent or kit for screening the acute high altitude reaction susceptible persons. The invention further provides a kit for detecting hsa-miR-15b-5p molecules in plain plasma of a tested person through a real-time fluorescent quantitative PCR method, and the onset risk of the acute high altitude reaction is predicted through the expression level of the hsa-miR-15b-5p molecules. As a molecular marker, the microRNA has the characteristics of high specificity, high sensitivity, noninvasiveness, convenience andthe like, and is suitable for large-scale screening of the acute high altitude reaction susceptible persons in a plain.

Description

technical field [0001] The invention belongs to the field of molecular biotechnology and its application. The invention relates to the application and kit of hsa-miR-15b-5p as a molecular marker for acute altitude sickness susceptible persons. Check the risk of acute altitude sickness. Therefore, it can be used to assess the susceptibility of the human body to acute altitude sickness and reduce the risk of acute altitude sickness. Background technique [0002] Acute mountain sickness (AMS) occurs in plain people who have lived in low-altitude areas for a long time, 1-3 days after entering the plateau quickly without adapting to the new environment, including headache, dizziness, fatigue, insomnia , Emotional restlessness, flatulence, diarrhea and vomiting and a series of symptoms, among which severe headache is a typical symptom of AMS. In geography, a vast area with an altitude higher than 500m and relatively flat or undulating terrain is called a plateau. In medicine, a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
Inventor 高钰琪徐刚黄河鄂国基孙滨达李鹏柯贤峰董华平陈建张二龙
Owner ARMY MEDICAL UNIV