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A kind of synergist for controlling cotton bollworm with bt protein and its combined use with bt protein to prevent and control cotton bollworm

A technology of cotton bollworm and bt protein, applied in the direction of pesticides, botanical equipment and methods, applications, etc., can solve the problems of not finding and applying enhanced Bt protein

Active Publication Date: 2021-03-23
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the Bt proteins used to control cotton bollworm and delay resistance are the mixed use and combination of Bt proteins. Since most of the Bt proteins used to control Lepidoptera pests have similar protein spatial structures, cross-resistance is easy to occur, and has not been found yet. and application of synergists that enhance Bt protein activity

Method used

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  • A kind of synergist for controlling cotton bollworm with bt protein and its combined use with bt protein to prevent and control cotton bollworm
  • A kind of synergist for controlling cotton bollworm with bt protein and its combined use with bt protein to prevent and control cotton bollworm
  • A kind of synergist for controlling cotton bollworm with bt protein and its combined use with bt protein to prevent and control cotton bollworm

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Experimental program
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Effect test

Embodiment 1

[0030] The bioassay was carried out on the 2nd instar cotton bollworm larvae, and Table 1 shows the mortality rate of cotton bollworm under different treatments.

[0031] Table 1

[0032]

[0033] It can be seen from Table 1 that the 2nd instar cotton bollworm can grow normally under the treatment of 10 μM, 20 μM and 50 μM FK506 concentrations, and has no effect on growth and development. 2 μg / mL Cry1Ac caused 50% of the 2nd instar cotton bollworm to die, and after correcting the mortality rate, the mortality rate was 41.46%; 2 μg / mL Cry1Ac protein was mixed with different concentrations of FK506 (10 μM, 20 μM and 50 μM) Finally, FK506 combined with Cry1Ac at 50 μM can cause 91.87% of the actual observed corrected mortality of cotton bollworm, see figure 1 , predicting that 50 μM FK506 mixed with 2 μg / mL Cry1Ac, the predicted adjusted mortality rate should be 42.18%. The observed adjusted mortality rate was significantly higher than the predicted adjusted mortality rate ...

Embodiment 2

[0035] For Cry2Ab control of cotton bollworm, its method and implementation, refer to Example 1, wherein the concentration of Cry2Ab is 10 μg / mL, see Table 1. At this dose, the mortality rate of cotton bollworm larvae was about 15.85%, and the corrected mortality rate was 14.63%. Cry2Ab at 50 μM can cause 75.61% observed corrected mortality of cotton bollworm, predicted 50 μM FK506 mixed with 2 μg / mL Cry1Ac, predicted corrected mortality should be 16.61%. The actual death rate was significantly higher than the predicted death rate ( P =0.0022) see figure 2 , indicating that 50 μM FK506 can significantly synergize Cry2Ab against 2nd instar larvae of cotton bollworm.

[0036] It can be seen that the mixed use of 50 μM FK506 of the present invention and two different Bt proteins can play a significant synergistic effect, which will provide an effective synergist for the control of cotton bollworm, and can even be applied to cotton bollworm resistance management.

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Abstract

The invention relates to a synergist for Bt protein preventing and treating cotton bollworm and a method for preventing and treating cotton bollworm by jointly using the synergist and the Bt protein.When the synergist is respectively added into two Bt proteins, the insecticidal activity of Bt protein can be obviously promoted. The synergist is tacrolimus, with a molecular formula of C44H69NO12 and molecular weight of 804.03. The method for preventing and treating cotton bollworm by jointly using the synergist and the Bt protein comprises the step of mixing 2-10ug / mL Bt protein and 10-50uM synergist for preventing and treating cotton bollworm. The synergist found by the invention is capable of promoting the control efficiency of Cry1Ac and Cry2Ab to cotton bollworm and increasing fatalityrate, is capable of effectively delaying resistance and can be applied to treatment for Bt resistance of cotton bollworm.

Description

technical field [0001] The invention relates to the field of toxicological activity of pest control, in particular to a synergist for controlling cotton bollworm with Bt protein and a method for controlling cotton bollworm by using it in combination with Bt protein. Background technique [0002] Cotton bollworm belongs to Lepidoptera Noctuidae Helicopteridae. It is an important agricultural pest distributed worldwide, harming more than 100 crops such as cotton, corn, and vegetables. In northern my country, cotton bollworms generally have four generations a year. The first generation of cotton bollworm harms wheat, emerges in the wheat field, and the adults transfer to the cotton field in flowering stage to lay eggs. The second, third and fourth generations mostly occur on summer crops such as cotton, corn, soybean, peanut and vegetables. The outbreak of cotton bollworm in the early 1990s caused huge economic losses to my country's national economy. In order to effectively ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/90A01N61/00A01P7/04
Inventor 魏纪珍安世恒席玉强刘晓光杜孟芳关若冰
Owner HENAN AGRICULTURAL UNIVERSITY
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