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A kind of oleanane type triterpene saponin and its extraction and separation method and application

A technology of oleanane type and triterpene saponins, which is applied in the field of medicine and can solve the problems of few reports on the chemical components of rhizomes

Active Publication Date: 2021-07-02
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Uighur medicine reports that in addition to the use of Nigella sativa as medicine, its rhizome can also be used as medicine, but there are few reports on the chemical composition of its rhizome

Method used

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  • A kind of oleanane type triterpene saponin and its extraction and separation method and application
  • A kind of oleanane type triterpene saponin and its extraction and separation method and application
  • A kind of oleanane type triterpene saponin and its extraction and separation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Isolate and prepare the target compound (abbreviated NGTS) from Nigella glandularis, the method is as follows:

[0034] The raw material is Nigella nigularia collected from Urumqi, Xinjiang. Take 20 kilograms of the rhizome of Nigella glandularis, cut it into small sections with a guillotine, add 100 liters of 70% ethanol to carry out reflux extraction, each time for 2 hours, a total of Extract 3 times. The extracts were combined, and ethanol was recovered under reduced pressure to obtain 2.5 kg of extract. Disperse the extract in 10 liters of distilled water, extract 3 times with an equal volume of petroleum ether, discard the petroleum ether layer, then extract 3 times with an equal volume of water-saturated n-butanol, recover the n-butanol layer, and obtain 225 grams of total saponins. The total saponins were separated and purified with a silica gel chromatography column, and the mixed solvent gradient system of chloroform-methanol-water with a volume ratio of 20:1:...

Embodiment 2

[0036] Isolate and prepare the target compound (abbreviated NGTS) from Nigella glandularis, the method is as follows:

[0037] The raw material is Nigella nigularia collected from Urumqi, Xinjiang. Take 10 kg of the rhizome of Nigella glandularis, cut it into small sections with a guillotine, add 100 liters of 70% ethanol to carry out reflux extraction, each time for 1 hour, a total of Extract 3 times. The extracts were combined, and ethanol was recovered under reduced pressure to obtain 1.3 kg of extract. The extract was dispersed in 5 liters of distilled water, extracted three times with an equal volume of petroleum ether, discarded the petroleum ether layer, then extracted three times with an equal volume of water-saturated n-butanol, and recovered the n-butanol layer to obtain 116 grams of total saponins. The total saponins were separated and purified with a silica gel chromatography column, and the mixed solvent gradient system of chloroform-methanol-water with a volume ...

Embodiment 3

[0039] Isolate and prepare the target compound (abbreviated NGTS) from Nigella glandularis, the method is as follows:

[0040] The raw material is Nigella nigularia collected from Urumqi, Xinjiang. Take 30 kg of the rhizome of Nigella glandularis, cut it into small sections with a guillotine, add 100 liters of 70% ethanol for reflux extraction, each time for 2 hours, a total of Extract 3 times. The extracts were combined, and ethanol was recovered under reduced pressure to obtain 3.6 kg of extract. Disperse the extract in 10 liters of distilled water, extract 3 times with an equal volume of petroleum ether, discard the petroleum ether layer, then extract 3 times with an equal volume of water-saturated n-butanol, recover the n-butanol layer, and obtain 268 grams of total saponins. The total saponins were separated and purified with a silica gel chromatography column, and the mixed solvent gradient system of chloroform-methanol-water with a volume ratio of 20:1:0~6.5:3.5:1 was ...

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Abstract

The invention discloses an oleanane-type triterpene saponin and its extraction and separation method and its application. It is confirmed by spectral technology that the oleanane-type triterpene saponin is a new kind isolated from Nigella nigella for the first time. compound. The invention discloses a method for extracting and separating the above-mentioned oleanane-type triterpene saponins from Nigella nigellae. The total saponins are obtained after preliminary extraction and separation by 70% ethanol, and the total saponins are obtained after passing through a silica gel chromatographic column. The crude triterpene saponins were separated and purified by Sephadex LH‑20 gel column and high performance liquid chromatography, and the target compound NGTS was successfully separated. The present invention also discloses for the first time that the above-mentioned oleanane-type triterpene saponins have anti-tumor effects, and in vitro anti-tumor experiments have found that the compounds have obvious inhibitory effects on gastric cancer cells and glioma cells, indicating that they can be further used as new anti-tumor compounds. Drug research and development.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to an oleanane-type triterpene saponin, an extraction and separation method and application thereof. Background technique [0002] Nigella glandulifera, also known as Nigella nigella, is a plant of the genus Nigella (Ranunculaceae) in the family Ranunculaceae. It is mainly produced in Xinjiang and is a unique plant resource in Xinjiang. Its seeds are commonly used medicinal materials by the Uighurs. , often used for chest tightness, lack of gas, breast swelling, reduced milk, tinnitus, hot shower, stone shower, amenorrhea embolism. In recent years, there have been some reports on the chemical constituents and pharmacological effects of Nigella pilosae, mainly including saponins, flavonoids, alkaloids and other biologically active ingredients, which have anti-oxidation, anti-inflammation, immune regulation, anti-tumor, hypolipidemic, etc. pharmacological effects. Uighu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00A61P35/00A61K31/704
Inventor 姚敏娜汤海峰陆云阳张伟李锐莉李韦韦冯颖达高凯马宁丁一文爱东杨志福
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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