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pH-responsive magnetic nanoparticle assembly and its preparation method and application

A magnetic nanoparticle and assembly technology, which can be applied in preparations for in vivo tests, pharmaceutical formulas, emulsion delivery, etc., can solve the problems of not being obvious enough, not having intelligent response, and high toxicity

Active Publication Date: 2020-10-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the magnetic resonance nano-contrast agents in the prior art often have insufficient contrast, high toxicity, and do not have the function of intelligent response

Method used

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  • pH-responsive magnetic nanoparticle assembly and its preparation method and application
  • pH-responsive magnetic nanoparticle assembly and its preparation method and application
  • pH-responsive magnetic nanoparticle assembly and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1: Preparation of iron oxide nanoparticles

[0052] Weigh 10.8g ferric chloride (FeCl 3 ·6H 2 (2, 40mmol) and 36.5g sodium oleate (120mmol) were placed in a 500mL round bottom flask, 80mL of absolute ethanol, 60mL of deionized water, and 140mL of n-hexane were added as solvents, in a 70°C oil bath, and during the reaction Maintain a uniform stirring speed and react for 4 hours. After the reaction was completed, 30 mL of distilled water was used for extraction, the lower aqueous phase was discarded, and the operation was repeated three times. After the extraction was completed, n-hexane, a small amount of residual ethanol and water were removed by rotary evaporation to finally obtain the oil-iron complex in the form of waxy solid.

[0053] Accurately weigh 1.8g (2mmol) of the oil-iron complex and place it in a dry 50mL three-necked flask, then add 0.57g (2mmol) of oleic acid and 1.61g (6mmol) of oleyl alcohol, and add 10g of diphenyl ether as a reaction sol...

Embodiment 2

[0055] Example 2: Iron Oxide Nanoparticle Ligand Exchange

[0056] Weigh 0.5 g of 2-bromoisobutyric acid and 0.05 g of citric acid and place them in a 50 mL round bottom flask, add 15 mg of the synthesized iron oxide nanoparticles, and then add a mixed solution of chloroform and dimethylformamide (DMF) (50 / 50v / v, 15mL) dissolved the reactant, and stirred overnight in an oil bath at 30°C. Precipitate with ether, wash the precipitate twice with ether, and disperse the iron oxide nanoparticles exchanged with 2-bromoisobutyric acid in dimethylformamide for use.

Embodiment 3

[0057] Example 3: Polyethylene glycol modified iron oxide nanoparticles after ligand exchange

[0058] Weigh 50 mg of polyethylene glycol-amino (Shanghai Ziqi Biological Co., Ltd.), dissolve it in dimethylformamide, and add it to the dimethylformamide solution containing 10 mg of ligand-exchanged iron oxide nanoparticles. to 5 mL, stirred overnight at room temperature, and dispersed in deionized water after centrifugation to obtain polyethylene glycol-modified iron oxide nanoparticles.

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Abstract

The invention relates to a pH responsive magnetic nanoparticle assembly comprising magnetic nanoparticles, water-soluble macromolecules modified on the surface of the magnetic nanoparticles and i-motif DNA modified on the surface of the magnetic nanoparticles by a short single strand DNA. The assembly not only can show a bright T1 contrast effect in a tumor site, but also shows a dark T2 contrasteffect in a normal tissue site, and achieves double contrast. The invention also relates to a preparation method of the pH responsive magnetic nanoparticle assembly and an application of the assemblyin preparation of a magnetic resonance nanocontrast agent. The preparation method has controllable reaction conditions, and the product has uniform size, good morphology and good clinical transformation possibility.

Description

technical field [0001] The invention relates to the assembly of inorganic nanometer materials, in particular to a pH-responsive magnetic nanoparticle assembly and its preparation method and application. Background technique [0002] In the global disease, the death rate of cancer ranks second, second only to cardiovascular disease. However, for most solid tumors, since most patients are already in the advanced stage when they are diagnosed, they miss the best opportunity for treatment, so the overall prognosis is poor. More than 60%. Therefore, the early diagnosis and treatment of tumors has great clinical significance. [0003] Magnetic nanoparticles have attracted much attention in the field of MR imaging due to their own magnetic properties. Among them, magnetic nanoparticles such as iron oxide nanoparticles, sodium gadolinium fluoride, and manganese oxide benefit from their excellent biocompatibility and biosafety, and have been widely studied and applied clinically. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/18A61K49/10A61K49/12
Inventor 凌代舜鲁敬雄李方园汪瑾
Owner ZHEJIANG UNIV
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