A multifunctional carrier based on cell-derived vesicles in body fluids, its preparation method and application
A multifunctional, membranous vesicle technology, applied in the field of new biological and pharmaceutical dosage forms and preparations, which can solve the problems of photobleaching, limited effect, weak fluorescent signal, etc.
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Embodiment 1
[0114] A method for preparing a multifunctional carrier labeled with ultra-small manganese magnetic near-infrared quantum dots (Mn-NIR-QDs) based on cell-derived vesicles in body fluids, the steps of which are:
[0115] 1. Use vacuum blood collection tubes containing EDTA-K2 anticoagulant to collect peripheral blood samples, transfer them to centrifuge tubes, and centrifuge at 1800 or 1900 or 2000 or 2100 or 2200 g for 18 or 19 days at 3 or 4 or 5 °C or 20 or 21 or 22min, take the supernatant, centrifuge again at 1800 or 1900 or 2000 or 2100 or 2200g at 3 or 4 or 5°C for 18 or 19 or 20 or 21 or 22min, get the supernatant, and then Continue to centrifuge at 48000 or 49000 or 50000 or 51000 or 52000 g for 60 min at 4 or 5°C, resuspend the obtained pellet with sterile PBS, and freeze the resuspended liquid at -80°C to obtain blood-derived particles.
[0116] 2. Add 20 μg (50 μL) of blood-derived microparticles / 50 μL of ultra-small manganese magnetic near-infrared quantum dots (Mn...
Embodiment 2
[0124] Application of a multifunctional carrier labeled with ultra-small manganese magnetic near-infrared quantum dots (Mn-NIR-QDs) based on cell-derived vesicles in body fluids in the preparation of gene therapy drugs and chemotherapy drugs for the treatment or prevention of oral cancer and oral cavity The application in the early diagnosis of cancer, its steps are as follows:
[0125] 1. Use vacuum blood collection tubes containing EDTA-K2 anticoagulant to collect peripheral blood samples, transfer them to centrifuge tubes, and centrifuge at 1800 or 1900 or 2000 or 2100 or 2200 g for 18 or 19 days at 3 or 4 or 5 °C or 20 or 21 or 22min, take the supernatant, centrifuge again at 1800 or 1900 or 2000 or 2100 or 2200g at 3 or 4 or 5°C for 18 or 19 or 20 or 21 or 22min, get the supernatant, and then Continue to centrifuge at 48000 or 49000 or 50000 or 51000 or 52000 g for 60 min at 4 or 5°C, resuspend the obtained pellet with sterile PBS, and freeze the resuspended liquid at -80...
Embodiment 3
[0133] A multifunctional carrier based on cell-derived vesicles in body fluids, labeled with ultra-small manganese magnetic near-infrared quantum dots (Mn-NIR-QDs) and loaded with gene therapy fragments (survivin-siRNA) in the preparation of treatment or prevention of oral cavity The application of cancer chemotherapy drugs or gene therapy drugs, the steps are:
[0134] 1. Use vacuum blood collection tubes containing EDTA-K2 anticoagulant to collect peripheral blood samples, transfer them to centrifuge tubes, and centrifuge at 1800 or 1900 or 2000 or 2100 or 2200 g for 18 or 19 days at 3 or 4 or 5 °C or 20 or 21 or 22min, take the supernatant, centrifuge again at 1800 or 1900 or 2000 or 2100 or 2200g at 3 or 4 or 5°C for 18 or 19 or 20 or 21 or 22min, get the supernatant, and then Continue to centrifuge at 48000 or 49000 or 50000 or 51000 or 52000 g for 60 min at 4 or 5°C, resuspend the obtained pellet with sterile PBS, and freeze the resuspended liquid at -80°C to obtain bloo...
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