Strain for degrading phenanthrene and application thereof in soil remediation

A technology for bacterial strains and polluted soil, applied in the field of environmental microbiology, can solve the problem of long degradation cycle of bacterial strains, and achieve excellent degradation effect, fast and efficient degradation effect.

Active Publication Date: 2019-06-25
CHINA UNIV OF MINING & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the degradation cycle of many strains is

Method used

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  • Strain for degrading phenanthrene and application thereof in soil remediation
  • Strain for degrading phenanthrene and application thereof in soil remediation
  • Strain for degrading phenanthrene and application thereof in soil remediation

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0031] Example 1 Enrichment culture, purification, screening and identification of the strain of the invention

[0032] (1) Medium

[0033] (I) MSM medium: NaN03 4.0g, NH4Cl2 2.0g, KH2P04 1.5g, Na2HP040.5g, CaCl20.01g, MgCl2 0.2g, 1ml trace element solution, 1L deionized water, adjust to pH=7.0, kill at 121°C Bacteria 20min. (A certain amount of phenanthrene is added during use, and because phenanthrene is semi-volatile, add phenanthrene on the ultra-clean workbench after the medium is sterilized.)

[0034] (II) Selection medium (solid medium): NaN03 4.0g, KH2P04 1.5g, Na2HP04 0.5g, CaCl20.01g, MgCl2 0.2g, agar 20.0g, trace element solution 1ml, deionized water 1L, pH=7.0, Sterilize at 121°C for 20 minutes.

[0035] (III) Enrichment medium: peptone 10.0g, yeast extract 5.0g, NaCl 10.0g, dissolved in 1L deionized water, adjusted to pH=7.0 with NaOH and HCl, and sterilized at 121°C for 20min.

[0036] (2) Enrichment culture and purification of strains

[0037] Measure 10ml of the sludge...

Example Embodiment

[0049] Example 2 Cultivation of strain Pseudomonas sp. Lphe-2

[0050] (1) Optimization of the cultivation conditions of the strain of the invention

[0051] Seed solution preparation: Take the preserved slant medium and place it at room temperature for 2 hours, and use a sterile dropper to suck up 0.5 ml of inorganic salt medium (the composition of the inorganic salt medium: NaNO 3 4.0g, NH 4 Cl 2 2.0g, KH 2 PO 4 1.5g, Na 2 HPO 4 0.5g, CaCl 2 0.01g, MgCl 2 0.2g, 1ml trace element solution, 1L deionized water, adjust pH=7.0, sterilize at 121°C for 20min; composition of the trace element solution: FeSO 4 ·7H 2 O 2g, ZnSO 4 ·7H 2 O 0.03g, H 3 BO 3 0.3g, CoCl 3 ·6H 2 O 0.3g, NiCl 2 ·6H 2 O0.02g, Na 2 MoO 4 ·2H 2 O 0.03g, deionized water 1L, adjust pH=7.0. ) Is dripped onto the inclined surface, and after slight shaking, 0.2ml of the bacterial suspension is taken on the solid medium and streaked and cultured for 96h. The strains with good growth conditions were picked from the so...

Example Embodiment

[0060] Example 3 Application of strain Pseudomonas sp.Lphe-2 in the remediation of soil contaminated by phenanthrene and pyrene

[0061] The soil samples used in this experiment were collected in the garden on the south side of the west gate of the School of Environmental Monitoring, China University of Mining and Technology, and the surface vegetation was removed after sampling by the five-point method. After the collected soil samples are naturally air-dried, impurities such as stones and branches are removed and passed through a 2mm sample sieve. Crush the soil> 2mm and pass it through a 2mm sample sieve, mix well, and set aside.

[0062] The air-dried and sieved soil samples were sent to the Red Soil Station of the Chinese Academy of Sciences for physical and chemical properties determination. Specifically, it includes six indicators of pH, organic carbon, total nitrogen, total phosphorus, available phosphorus and available potassium. The mud preparation method of each test g...

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Abstract

The invention discloses a strain for degrading phenanthrene and application thereof in soil remediation, and belongs to the field of environmental microbiology. The strain is named pseudomonas sp. Lphe-2, belonging to pseudomonas, was deposited in the Guangdong Provincial Center for Microorganisms and Species Collection under the accession number GDMCC No: 60603. The strain is a gram-negative bacterium, the color is milky white, the shape is round, the edge is neat, the texture is viscous and the surface is smooth. Under the scanning electron microscope, it can be seen that the strain Lphe-2 is mostly in short rod shape, with a small number of spherical and long rods. It can be seen that there is a long rod shape with a splitting tendency, which is determined to be a bacillus. The strain has the function of degrading PAHs efficiently and can be used for the repair of polycyclic aromatic hydrocarbons. The strain Lphe-2 has a broad spectrum of PAHs degradation, and has a good degradationeffect on the actual PAHs contaminated soil.

Description

technical field [0001] The invention relates to the field of environmental microbiology, in particular to a phenanthrene-degrading bacterial strain and its application in soil restoration. Background technique [0002] Combustion-derived residues (pyro-genic) contain relatively large amounts of high molecular weight PAHs. For example, sites where wood burning, gasification / liquefaction of fossil fuels, carbon production and use occur are often polluted with high concentrations of fluoranthene, pyrene, benzene and benzo[a]pyrene. Since polycyclic aromatic hydrocarbons are the main components of creosote (PAHs accounted for 85% by mass), industrial activities related to the production and use of coal tar or bitumen will lead to the emission of large amounts of phenanthrene, fluoranthene and pyrene. Coke production is usually associated with materials containing anthracene, phenanthrene and benzo[a]pyrene. [0003] Soil is one of the important components of the natural enviro...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/38
Inventor 毛缜姬丹吕蒙蒙贺建彭思涵
Owner CHINA UNIV OF MINING & TECH
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