Method for alkalizing silica gel G plate in thin-layer chromatography identification of licorice traditional Chinese medicine preparation
A traditional Chinese medicine preparation and thin-layer chromatography technology, which is applied in the field of medical detection, can solve problems such as poor separation of chromatographic spots, and achieve the effects of easy observation, saving time and cost, and accurate identification results.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0024] A kind of alkalization method of silica gel G plate in thin-layer chromatographic identification of licorice traditional Chinese medicine preparation, comprises the following steps:
[0025] Step 1, the configuration of the alkalization solution: according to the ratio of sodium hydroxide solid: distilled water = 1:100, weigh 10 g of sodium hydroxide solid, add distilled water to dissolve and set the volume to 1000 ml, that is, prepare 1% sodium hydroxide solution;
[0026] Step 2, impregnating the silica gel G plate: put the silica gel G plate to be treated into the container containing the 1% sodium hydroxide solution described in step 1 and soak for 1 minute, and take out the silica gel G plate after 1 minute;
[0027] Step 3, activation of the silica gel G plate: the silica gel G plate described in step 2 is taken out and then air-dried naturally, and after drying, place the silica gel G plate on a heating plate at 105°C and heat for 15-60 minutes;
[0028] Step 4, ...
Embodiment 2
[0031] A kind of alkalization method of silica gel G plate in thin-layer chromatographic identification of licorice traditional Chinese medicine preparation, comprises the following steps:
[0032] Step 1, the configuration of the alkalization solution: according to the ratio of sodium hydroxide solid: distilled water = 1:100, weigh 10 g of sodium hydroxide solid, add distilled water to dissolve and set the volume to 1000 ml, that is, prepare 1% sodium hydroxide solution;
[0033] Step 2, impregnating the silica gel G plates: put the multiple silica gel G plates to be treated into the container containing the 1% sodium hydroxide solution described in step 1 and soak for 1 minute, and take out the silica gel G plates after 1 minute;
[0034] Step 3, activation of silica gel G plates: take out the multiple silica gel G plates described in step 2 and air dry them naturally. After drying, place the silica gel G plates on a heating plate at 105°C for 15 minutes, 30 minutes and 60 mi...
Embodiment 3
[0046] In order to prevent the above-mentioned experimental test solution of different batches from causing unnecessary influence on the present invention, the present embodiment adopts the same type of test solution of different batches to verify the experimental results again, and uses ordinary silica gel G plate as a contrast, The specific operation is as follows:
[0047] A kind of alkalization method of silica gel G plate in thin-layer chromatographic identification of licorice traditional Chinese medicine preparation, comprises the following steps:
[0048] Step 1, the configuration of the alkalization solution: according to the ratio of sodium hydroxide solid: distilled water = 1:100, weigh 10 g of sodium hydroxide solid, add distilled water to dissolve and set the volume to 1000 ml, that is, prepare 1% sodium hydroxide solution;
[0049] Step 2, impregnating the silica gel G plates: put the multiple silica gel G plates to be treated into the container containing the 1%...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More