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Method for producing doramectin by continuous feeding method

A doramectin and feeding technology, which is applied in the field of doramectin fermentation production, can solve the problems of difficulty in controlling dissolved oxygen, waste of energy, and large solid content in the culture medium.

Inactive Publication Date: 2019-07-02
JIANGSU WEI LING BIOCHEM TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, manufacturers who use starch as the carbon source in the medium add starch to the medium at one time, resulting in a large solid content of the medium and high viscosity in the early stage of fermentation, which makes it very difficult to control dissolved oxygen in the early stage of the fermentation process, and air needs to be added. Flow rate, stirring speed, waste of energy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] The invention provides a technical solution: 1. Take out part of the cornstarch from the cornstarch ratio of 150g / L in the original formula fermentation liquor and sterilize it in the feeding tank, monitor the total sugar content in the fermentation liquor every 24 hours, and the low At 50g / L, the sterilized cornstarch was added to keep the total sugar content in the fermentation broth at 30-50g / L. The specific operation is as follows: (the statistical data is the logarithmic growth period of 40 to 112 hours)

[0020] a: starch ratio 150g / L

[0021] cycle Fermentation broth viscosity (cp) Ventilation volume (VVM) Stirring speed (rpm) Dissolved oxygen (%) 40h ３１５ ０．５ 30 87.3 64h ６０１ ０．8 50 20.8 88h ８５６ 1.0 80 0 112h ８２３ 1.0 80 23.7

[0022] The solid content of this batch after sterilization was 11%, and the potency was 1157 μg / ml.

[0023] b: Starch ratio 120g / L

[0024] cycle Fermentation broth visc...

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Abstract

The invention discloses a method for producing doramectin by a continuous feeding method. The method comprises the steps of dividing the quantity of starch in doramectin fermentation culture mediums into two parts, directly adding a part of the starch into the culture mediums, and adding the other part of the starch into a fermenter in a continuous adding manner in the fermentation process. Through the adoption of the method, solid content and stickiness of the fermentation culture mediums can be greatly reduced, so that oxygen in the fermentation culture process can better dissolve in fermentation liquor, the ventilation quantity is greatly reduced in the early stage during fermentation under the condition of guaranteeing oxygen dissolving, stirring load is reduced, and energy resources are saved. The method is used in the fermentation production process.

Description

technical field [0001] The invention belongs to the fermentative production of doramectin, and relates to the adjustment of the doramectin medium formula and feeding method. Background technique [0002] The carbon sources used in the fermentation of doramectin at home and abroad are mostly starch, maltose, glucose, etc. Among them, manufacturers who use starch as the carbon source in the medium add starch to the medium at one time, resulting in a large solid content of the medium and high viscosity in the early stage of fermentation, which makes it very difficult to control dissolved oxygen in the early stage of the fermentation process, and air needs to be added. Flow rate, stirring speed, waste of energy. Contents of the invention [0003] The purpose of the present invention is to provide a method for producing doramectin by adopting a continuous feeding method, which provides a method with greatly reduced ventilation, reduced stirring load, and energy saving. After ...

Claims

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Application Information

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IPC IPC(8): C12P19/62
CPCC12P19/62
Inventor 不公告发明人
Owner JIANGSU WEI LING BIOCHEM TECH CO LTD