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Vertical multi-electrode impedance sensor for real-time monitoring of 3D tumor cell migration and preparation method thereof

A technology for real-time monitoring of tumor cells, applied in biochemical equipment and methods, material impedance, microbial measurement/inspection, etc., to achieve the effect of monitoring migration ability

Active Publication Date: 2019-07-02
ZHEJIANG UNIV
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Problems solved by technology

At present, there are only methods for monitoring 2D cell migration in the market, such as transmembrane / Boyden chamber, cell scratch experiment, cell isolation migration experiment and microfluidic technology; there is a lack of effective monitoring methods for 3D cell migration

Method used

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  • Vertical multi-electrode impedance sensor for real-time monitoring of 3D tumor cell migration and preparation method thereof
  • Vertical multi-electrode impedance sensor for real-time monitoring of 3D tumor cell migration and preparation method thereof
  • Vertical multi-electrode impedance sensor for real-time monitoring of 3D tumor cell migration and preparation method thereof

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Embodiment Construction

[0027] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, but the present invention is not limited.

[0028] In the present invention, the vertical counter electrode is used to detect the total impedance of the 3D cell gel group. Since the gel impedance value is basically unchanged, the total impedance value of the 3D cell gel group is mainly determined by the number and state of the cells. Based on this principle, we designed a vertical multi-electrode impedance sensor to detect 3D tumor cell impedance values ​​distributed in different spatial positions in the cell culture chamber. As time changes, 3D tumor cells will undergo cell migration, and the impedance values ​​at different sites will be different from those at the initial moment. According to the impedance values ​​detected by different electrodes, we drew 3D cell migration maps at different time points to evaluate the cell migration ability....

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Abstract

The invention discloses a vertical multi-electrode impedance sensor for real-time monitoring of 3D tumor cell migration and a preparation method thereof. According to the invention, a vertical multi-electrode impedance chip is processed by using an MEMS process; 3D culturing is carried out on tumor cells having migration abilities; one pair of vertical multi-electrode impedance chips is inserted into a 3D tumor cell culture chamber; and with changing of the number of 3D tumor cells, the impedance values detected by the multi-electrode impedance chips also change. Because of the migration characteristics of tumor cells, the numbers of tumor cells at different spatial locations in the same culture chamber change with time. The vertical multi-electrode impedance chips can simultaneously monitor the impedance values of 16 different positions in the same culture chamber and a 3D cell impedance hotspot map is drawn based on the impedance value, so that the real-time monitoring of 3D tumor cell migration is realized.

Description

technical field [0001] The invention relates to cell migration detection technology, in particular to a vertical multi-electrode impedance sensor and a preparation method for real-time monitoring of 3D tumor cell migration. Background technique [0002] Cell migration, also known as cell crawling or cell movement, is one of the normal functions of normal cells, and refers to the movement of cells after sensing a migration signal. The migration of tumor cells belongs to the malignant behavior of tumor cells. Tumor cells invade lymphatic vessels, blood vessels or body cavities from their original site. the same type of tumor. The metastatic characteristics of malignant tumors bring great harm to the diagnosis and treatment of patients. At present, there is a huge demand for the research and development of anti-metastasis drugs. Traditional 2D cells often lose their in vivo characteristics because their culture environment is far from the in vivo environment. In order to imp...

Claims

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Application Information

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IPC IPC(8): G01N27/02C12Q1/02C23C14/02C23C14/35C23C14/18C23C14/16C23C14/58
CPCC23C14/024C23C14/165C23C14/185C23C14/35C23C14/5873G01N27/02
Inventor 王平潘宇祥梁韬孔留兵顾陈磊邱勇
Owner ZHEJIANG UNIV
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