Micro-fluidic chip for analyzing and sorting tumor cell migration capability and preparation process thereof

Abstract

The invention discloses a micro-fluidic chip for analyzing and sorting tumor cell migration capability. The micro-fluidic chip comprises a cell sample introduction channel, a chemical concentration gradient channel, a migration channel and a micro hook; two branches of the cell sample introduction channel are connected with the chemical concentration gradient channel through the migration channel; the micro hook is arranged at the joint of the cell sample introduction channel and the migration channel; a cell sample introduction inlet is formed in the top end of the cell sample introduction channel; a compound sample introduction inlet is formed in the bottom end of the chemical concentration gradient channel; a cell sample introduction outlet is formed in the middle of the chemical concentration gradient channel; and a compound sample outlet is formed in the top end of the chemical concentration gradient channel. Data collection can be carried out on migration paths and migration forms of cells in real time, and the data are analyzed to obtain the migration capability of each cell, the individual difference between the migration capabilities and the ratio of strong / weak migration cell subgroups. In addition, cell subgroups with specific migration capabilities can be enriched by microfluidic operations respectively, for subsequent molecular biology inventions.

Description

technical field [0001] The invention relates to the technical field of cell migration ability analysis, in particular to a microfluidic chip for analysis and sorting of tumor cell migration ability and a preparation process. Background technique [0002] Cell heterogeneity (Heterogeneity) widely exists in the process of stem cell differentiation, tumorigenesis, drug metabolism and immune response. This means that in the cell population of the same source, the genome or function of each single cell is different. Scientists are increasingly realizing that cell biology in the so-called "average" state cannot meet the needs of function and mechanism discovery. These methods often cover up the differences between cells and hinder us from understanding the complexity of biological individuals in tumor metastasis. and heterogeneity. Therefore, it is very necessary to discover tumor cells from the single cell level. [0003] The current analysis methods for cell migration ability...

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Problems solved by technology

[0003] The current analysis methods for cell migration ability are mainly scratch experiments and transwell experiments. These methods can averagely measure the migration ability of a population of cells. Due to the average processing of the results, the experimental results may cover up the function of a single or a small number of cells. At the same time, due to the lack of corresponding cell recycling methods, it is difficult to target and analyze cells with specific migration phenotypes, and it is impossible to further explore the molecular mechanism behind cell migration

[0004] At present, single cell analysis techniques mainly include micromanipulation, flow cytometry and other methods, but these methods have technical problems such as cumbersome operation, mechanical damage to cells, and high price.

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