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Application of polypeptide C2ORF40MPF in preparing antitumor drugs

A technology of C2ORF40MPF and anti-tumor drugs, applied in the field of biomedicine, can solve the problems of different therapeutic targets and mechanisms of action, and achieve the effect of inhibiting proliferation

Active Publication Date: 2017-07-07
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the different pathogenesis, therapeutic targets and mechanism of action of different types of tumors, there is no report about the polypeptide C2ORF40MPF in the treatment of breast cancer and lung cancer.

Method used

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  • Application of polypeptide C2ORF40MPF in preparing antitumor drugs
  • Application of polypeptide C2ORF40MPF in preparing antitumor drugs
  • Application of polypeptide C2ORF40MPF in preparing antitumor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: the synthesis of polypeptide C2ORF40MPF

[0038] The amino acid sequence of the polypeptide C2ORF40MPF of the present invention is: Ser-Pro-Tyr-Gly-Phe-Arg-His-Gly-Ala-Ser-Val-Asn-Tyr-Asp-Asp-Tyr (SEQ ID NO.1), entrusted Synpeptide company for the synthesis of peptides.

[0039] In order to compare the actual effect of the tumor suppressor polypeptide C2ORF40MPF designed in the present invention, we scrambled its amino acid sequence and entrusted a company to synthesize it to obtain Scrambled C2ORF40 mimic peptide (ScrC2ORF40), whose sequence is Asp-Ala-Phe-Tyr-Tyr- Arg-Asn-Gly-Asp-His-Tyr-Pro-Val-Ser-Gly-Ser.

Embodiment 2

[0040] Embodiment 2: MTT experiment

[0041] 1. Experimental method:

[0042] at 37°C, 5% CO 2 Cultivate breast cancer cell lines BT549, MDAMB231, lung cancer cell lines A549, H1299 in a cell incubator with saturated humidity. When the cell density reaches about 70%, wash the cells once with PBS, digest with trypsin, then add the culture medium to suspend the cells and centrifuge Collect the cells, remove the supernatant, add culture medium to resuspend the cells and count the cells, divide the above cells into 2×10 3 The density per hole was inoculated in a 96-well plate; after cultivating for 24 hours, the culture medium was replaced, and culture medium containing different doses of polypeptides (polypeptide C2ORF40MPF and ScrC2ORF40 prepared in Example 1) were added respectively to continue culturing; according to the needs of the experiment, at different time points Add 10 μl of 5 mg / ml MTT solution to each well, continue to incubate for 4 hours, remove the supernatant o...

Embodiment 3

[0048] Embodiment 3: Colony formation experiment

[0049] 1. Experimental method

[0050] BT549 and MDAMB231 cells were respectively inoculated into 6 cm culture dishes, 500 cells / culture dish, culture medium was replaced after 96 h, and culture solution containing 100 μM ScrC2ORF40 or 100 μM C2ORF40MPF polypeptide was added respectively, and the culture was continued for about 2 weeks before the culture was terminated. Discard the culture medium, wash twice with PBS, add 5ml of methanol to fix the cells, remove the fixation solution after 15 minutes, add 1ml Giemsa and stain with the dye solution for 30 minutes. Then, gently wash away the dye solution with running water, place the culture dish in the air to dry, and finally count the number of clones under a microscope, according to the number of cells per clone: ​​30-50, 50-100, 100-200, >200 Count the number of clones respectively.

[0051] 2. Experimental results:

[0052] Experimental results such as figure 2 As show...

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Abstract

The invention discloses application of polypeptide C2ORF40MPF in preparing antitumor drugs. The polypeptide C2ORF40MPF has amino acid sequence as shown in SEQ ID No.1. According to the invention, the biological functions of the polypeptide C2ORF40MPF in tumor cell multiplication, migration, invasion, tumor formation, and so on are studies. In vitro experiments indicate that C2ORF40MPF can remarkably inhibit breast cancer and lung cancer cell multiplication capacity, and has remarkable inhibitory action on tumor cell migration and invasion. Meanwhile, in vivo experiments indicate that C2ORF40MPF can remarkably inhibit tumor growth in a nude mice allografted tumor model. More importantly, cell cycle experiments indicate that the polypeptide can exert the antitumor effect by inducing mitotic period retardation.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of polypeptide C2ORF40MPF in the preparation of antitumor drugs. Background technique [0002] In China, more than 1.6 million people are diagnosed with cancer every year, and about 1.2 million people die from cancer. Since the 1990s, breast cancer rates in China have grown at more than twice the global rate, particularly in urban areas. At present, breast cancer is the tumor with the highest incidence rate in Chinese women, and its mortality rate ranks sixth among tumor malignancies. It has become a major disease that endangers human health. Therefore, it is of great significance to strengthen the research on the treatment of breast cancer and explore new therapeutic targets. [0003] Lung cancer can be divided into small cell lung cancer and non-small cell lung cancer. It is one of the malignant tumors with the highest morbidity and mortality in the world....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/10A61P35/00
CPCA61K38/10
Inventor 魏光伟毛建华李超阳
Owner SHANDONG UNIV
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