Predicting risk model establishing method for mother serum AFP and freebeta-HCG to fetus hypospadias
A technology of hypospadias and method establishment, which is applied in the field of medical detection to achieve high sensitivity and specificity and great clinical value
Active Publication Date: 2019-07-05
杭州市妇产科医院
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However, using maternal serum alpha-fetoprotein (AFP) and free human chorionic gonadotropin (free β-subunit of human chorionic gonadotropin, free β-HCG) to predict fetal hypospadias has rarely been reported.
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[0019] Embodiment: A method for establishing a maternal serum AFP and free β-HCG risk model for fetal hypospadias prediction, the method comprising the following steps:
[0020] (1) According to whether there is fetal hypospadias, pregnant women are divided into a case group and a control group. The included case group refers to pregnant women who have been clinically confirmed to have live-born hypospadias. The control group is randomly selected pregnant women with normal fetal development at the same time. ;
[0021] (2) Time-resolved immunofluorescence method was used to detect the serum levels of AFP and free β-HCG in the two groups of pregnant women, AFP and free β-HCG were used to construct a risk calculation model, and the optimal cut-off value and the area under the curve (AUC) were determined according to the ROC curve;
[0022] (3) During detection, the time-resolved immunofluorescence method is used to detect the AFP and free β-HCG levels in the serum of the pregnan...
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The invention discloses a predicting risk model establishing method for mother serum AFP and freebeta-HCG to fetus hypospadias. The method comprises the following steps of (1), dividing pregnant womento a case set and a contrasting set according to existence of the fetus hypospadias; (2), detecting the AFP and freebeta-HCG levels of two sets of pregnant women by means of time-resolved fluoroimmunoassay; and (3), in detecting, detecting the AFP and the freebeta-HCG levels of the serum of the to-be-detected pregnant women by means of time-resolved fluoroimmunoassay, and performing screening bymeans of a risk calculating model which is constructed by the AFP and the freebeta-HCG. The predicting risk model establishing method is advantageous in that relatively high sensitivity and specificity are realized in middle pregnancy mother serum AFP and freebeta-HCG joint screening to the hypospadias fetus; and the mother serum AFP and freebeta-HCG can be new markers for predicting the fetus hypospadias; and the risk calculating model which is constructed according to the serum AFP and freebeta-HCG levels has relatively high clinical value for screening the fetus hypospadias.
Description
technical field [0001] The invention relates to the field of medical detection, and mainly relates to a method for establishing a risk model for fetal hypospadias prediction by maternal serum AFP and free β-HCG. Background technique [0002] Fetal hypospadias is a common congenital malformation of the male external genitalia characterized by an abnormal opening of the urethra to the penis, ventral scrotum, or perineum [1] , is one of the common deformities of the human genitourinary system. Although many literatures have reported that the etiology of hypospadias has been determined, for example, it is caused by single genes such as WT1, SF1, BMP4, etc. [2] , GSTT1HOXA4, IRX5, IRX6 and EYA1 and other genes [3] and maternally inherited polymorphisms in CYP1A1, GSTM1, and GSTT1 and the risk of hypospadias [4] Mixture of factors such as genetics and environmental factors such as endocrine disrupting chemicals [5-7] , smoking, low birth weight, maternal hypertension, and pree...
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IPC IPC(8): G16H50/30G16H50/50
CPCG16H50/30G16H50/50
Inventor 陈益明黄建霞胡文胜辛然卢莎张闻连结静莫蔚农顾琳媛李俐瑶褚雪莲张敏梅瑾
Owner 杭州市妇产科医院