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Aqua defense refreshing toner containing tremella spore exopolysaccharides

A technology of moisturizing toner and extracellular polysaccharides, which is applied in the field of skin care products, can solve the problems of high cost, low polysaccharide content, and restrictions on the application of polysaccharides, and achieve good anti-oxidation, good moisturizing, and excellent moisturizing effects

Active Publication Date: 2019-07-09
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, when polysaccharides are extracted from the fruiting body of Tremella fungus for utilization, on the one hand, it takes a long time to grow into the fruiting body of Tremella fungus; Useful polysaccharides are not only costly, time-consuming and laborious, but also extremely low in efficiency, which limits the application of Tremella fruiting body polysaccharides
Moreover, and there are few studies on tremella exopolysaccharides at present, there is a lot of room for exploration

Method used

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  • Aqua defense refreshing toner containing tremella spore exopolysaccharides
  • Aqua defense refreshing toner containing tremella spore exopolysaccharides
  • Aqua defense refreshing toner containing tremella spore exopolysaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The screening of embodiment 1 Tremella fungus spores

[0063] The best strains were screened from six tremella spores Tyc63, Y2, Y11, Tr01, Tr21 and Tr9901.

[0064] 1. Rejuvenation and cultivation of tremella spores

[0065] (1) Inoculate the strains stored in the -80°C refrigerator on the PDA solid medium for activation by streaking or spreading on a plate, and then culture them in a 25°C incubator at a constant temperature for 2-3 days;

[0066] (2) Select a single colony with a good shape on the medium, and inoculate a ring with an inoculation loop in the PDB medium in the ultra-clean workbench, 150r / min, 25°C shake flask culture for 3 days and produce to the logarithmic growth phase . Then take it out and place it in a refrigerator at 4°C for later use.

[0067] 2. Molecular identification of tremella spores

[0068] 1. Preparation of template DNA for PCR identification

[0069] (1) Take out the Tremella fungus spore liquid strain growing to the logarithmic ph...

Embodiment 2

[0101] Example 2 Screening of Tremella fungus spore strains with higher exopolysaccharide production and determination of fermentation cycle

[0102] 1. Screening of Tremella fungus spore strains with higher exopolysaccharide production

[0103] 1. Extraction and separation of Tremella spore exopolysaccharide

[0104] Embodiment 1 has determined that these four strains of Tyc63, Tr01, Tr21, and Tr9901 are Tremella spores, so screening is carried out with the objects of these four Tremella spores, and the specific experimental process is as follows:

[0105] Tremella spore fermentation broth→centrifugation→concentrate the supernatant, add 3V absolute ethanol to precipitate polysaccharides→precipitate→polysaccharide crude product→heat and dissolve in distilled water→centrifuge→supernatant→Sevage method to remove protein→centrifuge to take supernatant→3V ethanol Precipitation of polysaccharides→precipitation→freeze drying→refinement of polysaccharides

[0106] Steps:

[0107] ...

Embodiment 3

[0135] Example 3 Antioxidative Activity Analysis of Tremella Fungus Bacillus Exopolysaccharide

[0136] 1. The scavenging effect of tremella spore exopolysaccharide on hydroxyl free radicals

[0137] With reference to the experiment of people such as Yan Jun, measure (Yan Jun etc., 2005) contain 8.8mmol / L H in the reaction system 2 o 2 1mL, 9mmol / L FeSO 4 1mL, 9mmol / L salicylic acid-ethanol 1mL, polysaccharide solution 1mL with different concentrations, and finally add H 2 o 2 Start the reaction, react at 37°C for 0.5h, and measure the absorbance of each concentration at 510nm with distilled water as a reference. Considering the light absorption value of the polysaccharide itself, with 9mmol / L FeSO 4 1mL, 9mmol / L salicylic acid-1mL ethanol, 1mL polysaccharide solutions of different concentrations and 1mL distilled water were used as the background absorption value of the polysaccharide.

[0138] The formula for calculating the clearance rate is:

[0139]

[0140] I...

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Abstract

The invention discloses application of tremella spore exopolysaccharides to preparing aqua defense refreshing products, and an aqua defense refreshing toner containing tremella spore exopolysaccharides. The preparing process of the tremella spore exopolysaccharides includes the steps of firstly, taking supernate of tremella spore fermentation liquid, and adding absolute ethyl alcohol for precipitation; secondly, adding water to precipitates for dissolving, taking supernate, removing protein through a Sevage method, conducting centrifuging to obtain supernate, adding ethyl alcohol for precipitation, and freeze-drying precipitates to prepare the tremella spore exopolysaccharides. The purified exopolysaccharides have a quite good aqua defense effect, and even the defense effect is better thanthat of glycerinum; meanwhile, the exopolysaccharides have a quite good anti-oxidization effect and can be applied as aqua defense active components and anti-oxidization active components to preparing the skincare products; the prepared skincare products have a quite good aqua defense effect and meanwhile have a function of repairing cell injuries caused by UVB radiation.

Description

technical field [0001] The invention relates to the technical field of skin care products, in particular to a moisturizing toner containing tremella exopolysaccharide. Background technique [0002] Tremella polysaccharide is a kind of heteropolysaccharide extracted and purified from the fruiting body of Tremella fungus, submerged fermentation spore liquid, etc. According to its nature and source, it can be divided into neutral polysaccharides, acid polysaccharides, exopolysaccharides, cell wall polysaccharides and acid oligosaccharides. Five types of sugar can promote the body's immune function, inhibit the deterioration of tumors, reduce blood sugar and blood lipid levels, remove free radicals from the body and slow down the aging of the body, and at the same time protect the body from radiation damage. [0003] However, when polysaccharides are extracted from the fruiting body of Tremella fungus for utilization, on the one hand, it takes a long time to grow into the fruiti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/73A61K8/9728A61Q19/00A61Q19/08
CPCA61K8/73A61K2800/85A61Q19/00A61Q19/08A61K8/9728
Inventor 郭丽琼王久莹郑倩望林俊芳
Owner SOUTH CHINA AGRI UNIV
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