Synthetic method of polyaspartic acid derivative grafted with glycyl histidyl lysine

A technology of glycyl histidyl lysine acetate and glycyl histidyl lysine, which is applied in the field of preparation of polyaspartic acid derivatives, can solve application limitations and limited functionality of polyamino acids and other problems, to achieve the effect of stimulating skin tissue repair, simple and green synthesis steps, and increasing grafting rate

Active Publication Date: 2021-06-08
湖南黛薇尔新材料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limited functionality of polyamino acid alone, its application is also limited.

Method used

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  • Synthetic method of polyaspartic acid derivative grafted with glycyl histidyl lysine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Purification of glycyl histidyl lysine: choose 50 parts of crude glycyl histidyl lysine synthesized by liquid phase method and dissolve it in 500 parts of ultrapure water, and dissolve it with ultrasonic wave. After the solution is completely clarified, use Filter through a 0.35 μm microporous membrane and collect the filtrate. The obtained filtrate uses a strong cation exchange column to remove trifluoroacetic acid and some impurities, and converts it into an acetate solution. The obtained acetate solution was desalted and purified using a reverse-phase polymer column, and finally the target fraction was collected, concentrated by distillation under reduced pressure, and freeze-dried to obtain pure glycyl-histidyl-lysine.

[0028] Synthesis of polyaspartic acid salt solution grafted with glycyl histidyl lysine: add 100 parts of L-aspartic acid into the kneading reactor, raise the temperature to 175°C, and control it under the condition of vacuum degree -20KPa After 4 ...

Embodiment 2

[0030] Purification of glycyl histidyl lysine: choose 40 parts of crude glycyl histidyl lysine synthesized by liquid phase method and dissolve it in 400 parts of ultrapure water, and ultrasonically assist the dissolution. After the solution is completely clarified, use Filter through a 0.45 μm microporous membrane and collect the filtrate. The obtained filtrate uses a strong cation exchange column to remove trifluoroacetic acid and some impurities, and converts it into an acetate solution. The obtained acetate solution was desalted and purified using a reverse-phase polymer column, and finally the target fraction was collected, concentrated by distillation under reduced pressure, and freeze-dried to obtain pure glycyl-histidyl-lysine.

[0031] Synthesis of polyaspartic acid salt aqueous solution grafted with glycyl histidyl lysine: add 200 parts of L-aspartic acid into the kneading reactor, raise the temperature to 185°C, and control it under the condition of vacuum degree -35...

Embodiment 3

[0033] Purification of glycyl histidyl lysine: select 30 parts of crude glycyl histidyl lysine synthesized by liquid phase method and dissolve it in 300 parts of ultrapure water, and dissolve it with ultrasonic wave. After the solution is completely clarified, use Filter through a 0.45 μm microporous membrane and collect the filtrate. The obtained filtrate uses a strong cation exchange column to remove trifluoroacetic acid and some impurities, and converts it into an acetate solution. The obtained acetate solution was desalted and purified using a reverse-phase polymer column, and finally the target fraction was collected, concentrated by distillation under reduced pressure, and freeze-dried to obtain pure glycyl-histidyl-lysine.

[0034] Synthesis of polyaspartic acid salt aqueous solution grafted with glycyl histidyl lysine: add 120 parts of L-aspartic acid into the kneading reactor, raise the temperature to 195°C, and control it under the condition of vacuum degree -50KPa ...

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Abstract

A kind of synthetic method of the polyaspartic acid derivative of glycyl histidyl lysine grafting, it is based on glycyl histidyl lysine (GHK) tripeptide compound, L-aspartic acid As a raw material, phosphoric acid is synthesized as a catalyst. The synthesis method of the present invention is simple and easy to operate, innovatively coupling the grafting modification step and the polymerization step to complete in one reactor, achieving the purpose of simplifying the process steps and regulating the degree of polymerization and grafting rate. The polyaspartic acid derivative synthesized by histidyl lysine as a modifier is a multifunctional new biomedical product of polypeptide polymerization, which has high molecular weight, strong moisture retention, good coordination ability, and can promote cell growth. , anti-wrinkle and environmental bio-friendly advantages, can be used in cosmetics and health products as moisturizers, anti-wrinkle additives and pharmaceutical carriers or slow-release agents, the production process is green and environmentally friendly, and it is an economical polyaspartic acid product with high added value method of preparation.

Description

technical field [0001] The invention relates to a preparation method of a graft-modified polyaspartic acid derivative, in particular to a preparation method of a polyaspartic acid derivative using glycyl histidyl lysine as a modifier. Background technique [0002] Polyaspartic acid (PASP) is a new type of green polymer chemical developed in recent decades. It was awarded the first "Presidential Green Chemistry Challenge Award" by the Environmental Protection Institute of the United States for its excellent performance in industry and agriculture. Polyaspartic acid is a polyamino acid with a carboxylic acid side chain, which can not only biodegrade, but also have the functions of chelation and dispersion. Generally, the amino group and carboxyl group of aspartic acid monomer are dehydrated and condensed to produce peptides. It is a macromolecular polypeptide with a protein structure. Because of its excellent ion chelating ability and super hydrophilic ability, polyaspartic a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G73/10
CPCC08G73/1092
Inventor 吴有庭陈雷庄薇娜
Owner 湖南黛薇尔新材料有限公司
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