A functional ordered mesoporous polymer material and its preparation method and application

A polymer material and functionalization technology, applied in the field of materials, can solve problems such as narrow application range, and achieve the effects of low cost, fast response speed, good industrial production and application prospects

Active Publication Date: 2022-04-01
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The material is not further loaded with enzymes and has a narrow range of applications

Method used

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  • A functional ordered mesoporous polymer material and its preparation method and application
  • A functional ordered mesoporous polymer material and its preparation method and application
  • A functional ordered mesoporous polymer material and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] At 40°C, 1.5g F127(EO 106 PO 70 EO 106 ) was dissolved in 8.00g absolute ethanol and 1.00g water, stirred for 1h; 1.92g TiCl 4 Hydrolyze in 7.50 g of absolute ethanol and 0.50 g of water for 30 min; mix the two solutions, add 5 g of nitrogen source-modified soluble resole phenolic resin, and stir at 40 ° C for 2 h. Then spread the mixture evenly on the petri dish, and place it at room temperature for 24 hours to evaporate the organic solvent; finally transfer it to an oven at 100° C., and obtain the enzyme-loaded carrier after 24 hours. 200 mg of the obtained carrier was immersed in a 1 mg / mL cytochrome C solution, shaken for 24 hours, filtered, freeze-dried, and stored at -20°C. The obtained catalyst was used for the oxidation reaction of pyrogallol. Specific tests such as Figure 1-6 As shown, compared with the reaction catalyzed by free enzyme, the reaction rate of immobilized enzyme is about 2 times. And the immobilized enzyme generated 7.2μM product within 2m...

Embodiment 2

[0032] At 40°C, dissolve 1.5g F127 in 8.00g absolute ethanol and 1.00g water, stir for 1h; 1.92g TiCl 4Hydrolyze in 7.50 g of absolute ethanol and 0.50 g of water for 30 min; mix the two solutions, add 5 g of nitrogen source-modified soluble resole phenolic resin, and stir at 40 ° C for 2 h. Then spread the mixture evenly on the petri dish, and place it at room temperature for 24 hours to evaporate the organic solvent; finally transfer it to an oven at 100° C., and obtain the enzyme-loaded carrier after 24 hours. 200 mg of the obtained carrier was immersed in a 0.5 mg / mL hemoglobin solution, shaken for 24 hours, filtered, freeze-dried, and stored at -20°C. The obtained catalyst was used for the oxidation reaction of 4-AAP.

Embodiment 3

[0034] A method for preparing a functional ordered mesoporous polymer material, comprising the following steps: dissolving a nonionic surfactant in a mixed solution of water and an organic solvent to obtain a solution A, and mixing the solution A with a hydrolyzed titanium source , and add nitrogen source modified soluble resin for reaction, then volatilize water and organic solvent to obtain prepolymer; heat-cure the obtained prepolymer at low temperature, then immerse in enzyme solution for enzyme loading, and obtain functionalized organic ordered mesoporous polymer materials.

[0035] Among them, the nonionic surfactant adopts C 12 h 25 EO 23 , the mass concentration of nonionic surfactant is 0.5%, the reaction temperature is 15°C, and the reaction time is 4h; the titanium source is titanium tetrachloride; the nitrogen source is urea; the organic solvent is tetrahydrofuran; the soluble resin is phenolic resin; the enzyme solution The middle enzyme is cytochrome C. The l...

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Abstract

The present invention relates to a functional ordered mesoporous polymer material and its preparation method and application. The material comprises a mesoporous polymer and an enzyme loaded on the polymer. The mesoporous polymer utilizes a titanium source and Soluble resins are assembled with nonionic surfactants and nitrogen sources. Compared with the prior art, the loaded enzyme material prepared by the present invention has uniform pore size distribution, large specific surface area, high enzyme loading capacity, and can perform different enzyme-catalyzed reactions according to the catalytic properties of different enzymes. Compared with homogeneous enzyme-catalyzed reactions , the reaction speed is faster, and it is easy to separate from the reactant, realize multiple catalysis, conform to the theory of green chemistry, simple operation, simple and easy preparation method, and low cost.

Description

technical field [0001] The invention relates to the field of materials, in particular to a functionalized ordered mesoporous polymer material and its preparation method and application. Background technique [0002] Enzymes are nature's catalysts with high reactivity, selectivity and specificity under mild conditions. Enzyme catalysis is widely used in chemical, pharmaceutical and food industries. However, the use of enzymes in industrial applications is often hampered by their low operational stability, difficulty in recovery, and lack of reusability under operating conditions. Enzyme immobilization on solid supports can improve enzyme stability while facilitating separation and recovery while maintaining activity and selectivity. Enzyme immobilization refers to the attachment or integration of enzyme molecules into large structures by simple adsorption, covalent attachment, or encapsulation. In particular, multi-point connections between enzyme molecules and host materi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G83/00C12N11/089
CPCC08G83/008C12N11/08
Inventor 万颖杨灏天汪珂
Owner SHANGHAI NORMAL UNIVERSITY
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