Preparation method of polyurethane nanofiber immobilized enzyme

A nanofiber, immobilized enzyme technology, applied in the direction of being immobilized on/in an organic carrier, can solve the problems of reduced enzyme activity, no activity recovery performance, etc., to achieve high enzyme loading, easy recovery and repetition Use, high stability effect

Active Publication Date: 2012-06-27
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] Although in the current reports, the thermal stability and stability in organic solvents of enzymes immobilized on nanofibers have been improved to varying degrees, but none of them have the ability to restore activity. When used at high temperature, the activity of the enzyme will be continuously reduced

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  • Preparation method of polyurethane nanofiber immobilized enzyme
  • Preparation method of polyurethane nanofiber immobilized enzyme

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Embodiment Construction

[0020] Embodiments of the present invention are described below in specific forms, but the present invention is not limited to the embodiments.

[0021] 1) The preparation method of polyurethane nanofiber membrane: dissolving polyurethane in an organic solvent to prepare a solution of 10-20% by weight, adding 1-5% LiCl relative to the weight of polyurethane, fully dissolving, injecting into electrospinning In the silk device, electrospinning is carried out under the conditions of a voltage of 20-30 kV, a flow rate of the spinneret solution of 0.1-0.5 ml / hour, and a receiving distance of 10-20 cm to obtain polyurethane nanometer fibers with a fiber diameter of 100-500 nm. Fiber membrane: Dry the nanofiber membrane in an oven at 60° C. for 24 hours, and then wash it with deionized water for use.

[0022] 2) Use physical adsorption to adsorb the inactive protein on the surface of the polyurethane nanofiber membrane to modify it. The specific method is to put the polyurethane nano...

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Abstract

The invention provides a preparation method of polyurethane nanofiber immobilized enzyme, which is characterized by comprising the following steps: (1) taking polyurethane as a raw material, adopting N, N-dimethylformamide and tetrahydrofuran of a certain ratio or dimethylacetamide as solvent as solvent, adding LiCl of a certain proportion to prepare electrospinning solution, and preparing a polyurethane nanofiber through electrospinning; (2) absorbing inert protein onto the fiber surface through physical adsorption to obtain a surface modified polyurethane nanofiber; and (3) immobilizing enzyme on the surface modified polyurethane nanofiber through joint of covalent bonds with glutaraldehyde as a crosslinking agent. Due to the adoption of the method of immobilizing the enzyme on the polyurethane nanofiber, the invention can effectively improve the stability of the enzyme. In particular, the immobilized enzyme prepared in the invention can gradually restore a majority of activities thereof when being placed in the buffer solution again after inactivation in the organic solvent or inactivation at the high temperature. Therefore, the immobilized enzyme has better stability and longer service life in the organic solvent or at the high temperature. Moreover, the preparation process is simple.

Description

Technical field: [0001] The invention relates to a preparation method for immobilizing enzymes using polyurethane nanofiber membranes, and belongs to the field of biochemical engineering. Background technique: [0002] Enzymes immobilized on solid materials are the main form of biocatalyst application due to the advantages of easy catalyst recovery, more flexible reactor design and relatively simple product purification. People use various chemical or physical methods to immobilize enzymes on various inorganic or organic solid supports, or embed enzymes in hollow fibers or microcapsules, or cross-link enzymes through covalent bonds. Among many factors, the structure and surface properties of the support material, as well as the way the enzyme binds to the material, are critical to the performance of the immobilized enzyme. Researchers have been working on optimizing the structure of support materials to prepare more effective biocatalysts. Nanostructured materials can maxi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/08
Inventor 张松平苏志国柳春霞
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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